General Questions:

Where are you located?

The GSL is located on the second floor (room 2518) of Thomas Hall on the North Campus of NC State University.

What time can I stop by to drop off samples?

Our hours for sample drop-off are 9 AM until 4 PM Monday through Friday. We ask that if you want to stop by during lunch (12-1 PM) that you call ahead to confirm your plans (919-513-3882).

Can I schedule a meeting to discuss a project I had in mind?

We encourage you to discuss your sequencing project with us. . Please email or call Jenn (919-513-0738; jeschaff@ncsu.edu), Julie (919-513-3882; jabenson@ncsu.edu), or Cory (919-513-3882; cldashie@ncsu.edu) to set-up a time.

Do you accept projects from outside of NC State University? Do you accept projects from outside of the United States?

Yes. We have many customers from all over the country and around the world. Please call or email Jenn (919-513-0738; jeschaff@ncsu.edu) for questions about billing concerns.

Are your prices different for NC State customers, other universities, or for businesses?

All U.S. federally-funded parties are charged the same use rates. Other parties or businesses, please contact us to discuss pricing.

Single Tube and Full Plate Sanger (Capillary) Sequencing:

What concentration does my PCR product need to be at for Sanger (Capillary) Sequencing?

Please see our page on Sanger sequencing for additional details.

Do you add primers for Single-Tube Sequencing?

We can add M13F, M13R, T7, or Sp6 primers.

What size tubes do you prefer that I use for my samples?

Please bring your sample in 1.7mL tubes that are clearly labeled.

What time must I drop-off my sequencing samples if I wish same-day service?

If you would like your Single-Tube Sequencing results back the same day, your samples must be dropped-off at the GSL no later than 12 PM. We also appreciate it if you fill-out the Single-Tube Sequencing Form and send it to us by email the morning before you drop-off your samples.

When will I get my results?

If you drop your sample off before noon, you should have results before 5 PM. If you drop off your samples after noon, you will have them by 5 PM the next day.

Next Generation Sequencing:

How much DNA/RNA do I need for Next Generation Sequencing Library preps?

While it depends on the type of prep that you are interested in, a good starting point is 2 ug of DNA or RNA. If you are unable to prepare 2 ug of DNA or RNA from your sample, please contact the GSL. Mate Pair Library preps do require significantly more starting material.

What type of Next Generation Sequencing Services do you offer?

We currently have a MiSeq Illumina sequencer and an Ion Torrent Proton sequencer in house. We also offer HiSeq sequencing through BGI.

How do you recommend shipping RNA or DNA to your lab?

We recommend shipping DNA overnight with freezer packs. For RNA we recommend you perform an ethanol precipitation and ship the RNA overnight as a pellet in a 70% EtOH wash on dry ice.

Do you extract DNA or RNA for customers?

Yes. Please contact the GSL for more information.

Sample Submission for Bioanalyzer and PippinPrep:

What concentration should my sample be at for the Bioanalyzer?

For the DNA High Sensitivity Chip and for the RNA Pico chip, please bring your samples at a concentration of 1-5ng/ul. For the RNA nano chip, samples may be at 50-200ng/ul.

How much sample do I need to bring for the Bioanalyzer?

Please bring 3-5 ul of each sample at the appropriate concentration in a 1.7 mL tube labeled clearly.

How do I prepare my sample for the PippinPrep?

Pippen prep samples should be submitted in exactly 30ul of EB, TE, or water with the sample submission form.

Other Questions:

Do you let customers use the lab equipment?

Most of our equipment in used only by GSL staff, however, there are certain pieces of equipment that our customers can access after training. This includes (but may not be limited to) the Qiagen Tissue Lyser, Bekman Ultracentrifuge, ABI 9600-HT qRT-PCR, Covaris Ultrasonicator, and the BioTek Synergy H1 Hybrid Plate Reader. Prices for using the equipment varies, please contact the GSL for more information.

Do you set up qRT-PCR plates?

Currently our customers set up their own qRT-PCR plates and run the plates themselves. Training on the ABI 9600-HT qRT-PCR machine is required before you can access the calendar and sign-up to use the machine. Please contact the GSL for more information about training.

Do you train customers on how to prepare libraries?

We can train customers on how to prepare various Next Generation Libraries and also on RNA and DNA extraction techniques. Please contact the GSL for more information.

Do you train customers on how to use lab equipment?

Customers can be trained on certain pieces of equipment, including the Qiagen Tissue Lyser, Bekman Ultracentrifuge, ABI 9600-HT qRT-PCR, BioTek Synergy H1 Hybrid Plate Reader, and Covaris Ultrasonicator. Once the customer has finished training they may access a calendar where they can sign up to use the instruments. The Next Generation Sequencers, Bioanalyzer, and PippinPrep are used by GSL staff only.