Tracking of Approval and Approved Animal Numbers

All use of vertebrate animals at NC State requires prior IACUC approval of a protocol application. All animals must be accounted for in the application, including all neonates; e.g., pre-weanling animals euthanized to standardize litter size or to cull unwanted genotypes or sex, and animals that are expected to die as a result of a harmful genotype. Accounting for the use of larval stage animals (i.e. tadpoles, fish) is required. Use of prenatal, pre-hatch, or embryonic animals does not require listing in the application.

In accordance with federal regulations and university policy, the protocol application requires the Principal Investigator to provide a rationale for the numbers of animals to be used. It is implicit from these requirements that there be an institutional mechanism to ensure that the animal use is approved, and that the number of animals used is consistent with information in the approved protocol.

Each animal resource unit* must have written procedures in place for monitoring and documenting animal use. Procedures must include methods to ensure that animal use is covered by an active IACUC-approved protocol, and that the numbers of animals used do not substantially exceed those approved in the protocol. Animal resource units also should have procedures that allow retrieval of animal use numbers, by category of use, for completion of the USDA Annual Report or other annual accounting (e.g., AAALAC reporting).

*An animal resource unit is an administrative unit that, for the purposes of this policy, acknowledges and accepts responsibility for tracking IACUC approval and approved animal numbers. That unit could be an animal facility (e.g., Biological Resources Facility), a college-wide service unit (e.g., CVM Laboratory Animal Resources), a University Field Lab or Field Station, or an academic department.