The Andor Dragonfly 302 Spinning disk confocal is attached to a Nikon Eclipse Ti microscope and has a range of oil immersion objectives and TIRF (Total Internal Reflection Fluorescence) capability. The system is optimized for resolution and has two cameras: Zyla 4.2 Megapixel sCMOS camera and iXon Ultra 888 Ultra EM CCD Camera (1024 x 1024 pixels). It features Andor Micropoint (point scanner) with tunable pulsed N2 laser for organelle ablation and high precision photoactivation.
Laser Lines for Excitation
- 488 nm solid state smart diode laser, 50 mW
- 561 nm solid state smart OPSS laser, 100 mW
- 637 nm solid state smart diode laser, 140 mW
Dichroics and Emission Filters
- 405/488/561/640 quad excitation dichroic
- bandpass filter 525/50 nm
- bandpass filter 600/50 nm
- bandpass filter 725/40 nm
MicroPoint Galvo Position control; N2 pulsed laser (dye pump) 337 nm, 3 ns, 100 μJ, 20 Hz, with pulse controller, dyes: 551 nm and 590 nm for precise ablation/ photoactivation at 551 nm and 590 nm.
Andor iXon DU888U3-CS0-BV EM CCD camera, 1024 x 1024 pixels, pixel size 13 x 13 μm; Readout Rate 30 MHz; Frame Rate 26 – 9690 fps; QEmax > 90%.
Zyla PLUS 4.2 Megapixel sCMOS camera; QE 80%.
Mosaic 3 random access active illumination device, ~20 mm primary image plane field of illumination, pE-300 white LED illumination unit provides a broad spectrum of
illumination, UV peak at 400nm, covering the excitation bands of common fluorescent stains.
Microscope and Accessories
Nikon Eclipse TI inverted microscope with motorized x,y,z stage, additional piezo z-stage, joystick and touch panel docking control station. Perfect Focus attachment corrects for focus drifts during overnight time lapse experiments. Stage top incubation chamber with temperature, humidity and CO2 control (Okolab).
|Magnification||N.A. Immersion||Type||Working Distance|
|40x||1.3 oil||CFI Plan Fluor||0.2 mm|
|60x (phase)||1.4 oil||CFI Plan Apochromat||0.13 mm|
|60x (TIRF)||1.49 oil||CFI Apochromat (TIRF)||0.12 mm|
|100x (phase)||1.45 oil||CFI Plan Apochromat||0.13 mm|
Fusion and iQ3 for image acquisition. Imaris 9.0 for image analysis and data presentation.