The Zeiss LSM 710 is a laser scanning confocal microscope with advanced imaging capabilities offering high sensitivity with superior signal to noise ratio, spectral analysis with a 34 channel detector and the ability to monitor dynamic changes of multiple fluorophores. Modules for advanced confocal applications such as Fluorescence Energy Transfer (FRET), colocalization analysis, and Recovery After Photobleaching (FRAP) are available. The multi-time plug in allows automated 3D (x,y,z) or 4 D (x,y,z,t) scans of preprogrammed locations and 3D image stitching. Funding was provided in part by the National Science Foundation (MRI-0821192), the College of Agriculture and Life Sciences, the NCSU Biotechnology Program and the NCSU Office of the Vice Chancellor for Research.
Image & Video Gallery
Laser lines for excitation
- 405 nm diode laser (for DAPI, ALexa 350)
- 458 nm, 488 nm, 514 nm multiline argon laser
- 561 nm diode pumped solid state laser
- 633 nm HeNe laser
They can be flexibly selected with 2 flanking photomultiplier (pmt) channels and a 34 channel pmt array. The system can be opperated in lambda mode with 34 spectral detectors that collect the entire wavelength range in one scan or in channel mode with up to 10 simultaneously active detection channels that can be set as needed.
Differential interferrence contrast images
Differential interferrence contrast images can be acquired simultaneously and DIC prisms are available for all objectives except 5x.
Microscope and Accessories
The scanhead is attached to a Zeiss Axio Observer Z1 inverted microscope with motorized, programmable x,y,z stage, joystick and touch panel docking control station. A definite focus attachment corrects for focus drifts during overnight time lapse experiments. Temperature and CO2 control chambers are available.
|5x||0.16 dry||Plan-Apochromat||On request|
|25x||0.8 W/oil/glycerin||Plan-Apochromat||On request|
|40x||0.75 dry||Plan-Neofluar||On request|
|40x||0.95 dry||Plan-Apochromat||On request|
Please review these rules before reserving equipment, thank you.
Once approved to use the Zeiss LSM 710 confocal independently you can reserve time online by visiting the faces scheduling system. Log in entering your credentials. Use the “choose your schedule” pull down menu, navigate to ZEISS_LSM710 and click on your start time. Release the mouse button and click on the end time. Be aware that the time allowed per appointment and number of appointments may be restricted when the resource is in high demand. The current restrictions are listed below and are subject to change. Please use common sense and be considerate of other users. Appointments can currently be deleted 20h before start time. If you need to cancel at shorter notice you will be charged for the time reserved during peak time (see below) unless someone else that is not signed up at peak time can fill the slot. Please send an email notification to Eva Johannes and to a standby confocal user list.
Please note: The last person signed up for the day is responsible for powering the system off. If you are the last person on the schedule and do not need to use the confocal you still have to come in to switch the system off or find someone else to do it. If you ask someone by email make sure you receive confirmation. Leaving the confocal on overnight will result in a $50 fine.
Here are the current rules set in “faces” for resource “ZEISS_LSM710”:
- Prime time: Mo-Fr 9am-6pm
- Each appointment must begin less than 2 weeks after it is requested.
- Maximum time per appointment is 60 min (prime time) and 120 min (non-prime time).
- Number of appointments per day: 3, consecutive appointments allowed.
- Number of future appointments: 15.
- Appointment can be deleted 20h before start time.