{"id":1851,"date":"2017-04-18T12:05:16","date_gmt":"2017-04-18T16:05:16","guid":{"rendered":"https:\/\/research.ncsu.edu\/cmif\/?page_id=1851"},"modified":"2023-02-21T10:35:25","modified_gmt":"2023-02-21T15:35:25","slug":"zeiss-lsm-880","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/cmif\/equipment-list\/workstations\/zeiss-lsm-880\/","title":{"rendered":"Zeiss LSM 880 confocal workstation with Airyscan"},"content":{"rendered":"\n<p><strong>Please read the <a href=\"https:\/\/research.ncsu.edu\/cmif\/user-information\/facility-policy\/\">facility policy<\/a> and contact <a href=\"mailto:cmif_microscopy@ncsu.edu\">CMIF<\/a> to set up an appointment.<\/strong><\/p>\n\n\n\n<p>The Zeiss LSM 880 with Airyscan is the latest and most advanced model in the Zeiss LSM series of confocal laser scanning microscopes. Our configuration includes a 32 channel linear detector for spectral analysis and a 32 channel area detector (Airyscan) for point spread function modulation to increase S\/N and x, y, z resolution (1.7 fold). In addition our system features a beamshaper for the fast Airyscan mode which lets us acquire images 4 times faster with minor loss in spatial resolution. Both 32-channel detectors employ GaAsP detectors that are twice as quantum efficient as the traditional photo multiplier tubes. Modules for advanced confocal applications such as Fluorescence Energy Transfer (FRET), colocalization analysis, and Recovery After Photobleaching (FRAP) are available as well as advanced options for tiling and stitching and experimental design and automation. Funding was provided in part by the National Science Foundation (DBI-1624613) and the NCSU Office of the Vice Chancellor for Research.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Image and Video Gallery<\/h2>\n\n\n<div class=\"wp-block-ncst-photo-gallery has-gray-800-background-color\" id=\"ncst-photo-gallery-0\"><div class=\"ncst-photo-gallery__container\"><\/div><div class=\"ncst-photo-gallery__viewport\" aria-live=\"polite\"><div class=\"ncst-photo-gallery__viewport-reel\"><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"1\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600.jpg\" alt=\"\" data-id=\"2019\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-300x300.jpg\" class=\"wp-image-2019\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Arabidopsis guard cell vacuoles<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"2\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600.jpg\" alt=\"\" data-id=\"2020\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-300x300.jpg\" class=\"wp-image-2020\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Arabidopsis stomate vacuoles &#8211; 3D rendering<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"3\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600.jpg\" alt=\"\" data-id=\"2018\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-300x300.jpg\" class=\"wp-image-2018\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Arabidospsis root vacuoles<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"4\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600.jpg\" alt=\"\" data-id=\"2091\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-300x300.jpg\" class=\"wp-image-2091\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Watermelon placental tissue<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"5\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600.jpg\" alt=\"\" data-id=\"2023\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-300x300.jpg\" class=\"wp-image-2023\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Rat tendon cells grown on a scaffold<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"6\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"614\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600.jpg\" alt=\"\" data-id=\"2086\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600-293x300.jpg\" class=\"wp-image-2086\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600-293x300.jpg 293w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Fungal sclerotia<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"7\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600.jpg\" alt=\"\" data-id=\"2085\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-300x300.jpg\" class=\"wp-image-2085\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Fungal fruiting bodies<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"8\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"614\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600.jpg\" alt=\"\" data-id=\"2087\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600-293x300.jpg\" class=\"wp-image-2087\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600-293x300.jpg 293w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Fungal hyphae<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"9\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"601\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600.jpg\" alt=\"\" data-id=\"2022\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-300x300.jpg\" class=\"wp-image-2022\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-350x350.jpg 350w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Inside a developing blowfly ovary<\/figcaption><\/figure><figure class=\"ncst-photo-gallery-item\" data-gallery-item=\"10\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"606\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen.jpg\" alt=\"\" data-id=\"2102\" data-thumb-url=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen-297x300.jpg\" class=\"wp-image-2102\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen-297x300.jpg 297w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><figcaption class=\"ncst-photo-gallery__viewport-caption\">Tickseed stigma and pollen<\/figcaption><\/figure><\/div><\/div><div class=\"ncst-photo-gallery__container\"><div class=\"ncst-photo-gallery__navigation\"><div class=\"ncst-photo-gallery__controls\"><button class=\"prev\" disabled><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><path d=\"M448,216.66H205.64l103.87-97.57L256,64,64,256,256,448l53.51-53.51L205.64,295.34H448Z\"><\/path><\/svg><span class=\"sr-only\">Previous Image<\/span><\/button><span class=\"ncst-photo-gallery__progress\"><span class=\"ncst-photo-gallery__current-index\">1<\/span> of 10<\/span><button class=\"next\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><path d=\"M64,295.34H306.36L202.49,392.92,256,448,448,256,256,64l-53.51,53.51,103.87,99.15H64Z\"><\/path><\/svg><span class=\"sr-only\">Next Image<\/span><\/button><\/div><ul class=\"ncst-photo-gallery__thumbnails\"><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"1\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 1 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-300x300.jpg\" alt=\"\" class=\"wp-image-2019\" data-caption=\"Arabidopsis guard cell vacuoles\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_2D_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"2\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 2 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-300x300.jpg\" alt=\"\" class=\"wp-image-2020\" data-caption=\"Arabidopsis stomate vacuoles - 3D rendering\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-65_3D_modified_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"3\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 3 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-300x300.jpg\" alt=\"\" class=\"wp-image-2018\" data-caption=\"Arabidospsis root vacuoles\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/Image-27_2D_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"4\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 4 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-300x300.jpg\" alt=\"\" class=\"wp-image-2091\" data-caption=\"Watermelon placental tissue\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marlee_Trandel_01_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"5\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 5 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-300x300.jpg\" alt=\"\" class=\"wp-image-2023\" data-caption=\"Rat tendon cells grown on a scaffold\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/SherryCOL_RC_DAY14_10X_MIP-600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"6\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 6 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"293\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600-293x300.jpg\" alt=\"\" class=\"wp-image-2086\" data-caption=\"Fungal sclerotia\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600-293x300.jpg 293w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_02_600.jpg 600w\" sizes=\"auto, (max-width: 293px) 100vw, 293px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"7\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 7 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-300x300.jpg\" alt=\"\" class=\"wp-image-2085\" data-caption=\"Fungal fruiting bodies\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_01_600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"8\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 8 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"293\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600-293x300.jpg\" alt=\"\" class=\"wp-image-2087\" data-caption=\"Fungal hyphae\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600-293x300.jpg 293w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/08\/Marian_Luis_03_600.jpg 600w\" sizes=\"auto, (max-width: 293px) 100vw, 293px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"9\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 9 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-300x300.jpg\" alt=\"\" class=\"wp-image-2022\" data-caption=\"Inside a developing blowfly ovary\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-300x300.jpg 300w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-150x150.jpg 150w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600.jpg 600w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/06\/DR2-4-day-ovary_Subset-Megan-600-350x350.jpg 350w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/button><\/li><li class=\"ncst-photo-gallery-thumb\" data-gallery-item=\"10\"><button class=\"ncst-photo-gallery__thumb-btn\" aria-label=\"(Image 10 of 10)\"><img loading=\"lazy\" decoding=\"async\" width=\"297\" height=\"300\" src=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen-297x300.jpg\" alt=\"\" class=\"wp-image-2102\" data-caption=\"Tickseed stigma and pollen\" srcset=\"https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen-297x300.jpg 297w, https:\/\/research.ncsu.edu\/cmif\/files\/2018\/09\/coreopsis-stigma-with-pollen.jpg 600w\" sizes=\"auto, (max-width: 297px) 100vw, 297px\" \/><\/button><\/li><\/ul><\/div><\/div><div class=\"sr-only speak-region\" aria-live=\"polite\">Arabidopsis guard cell vacuoles<\/div><\/div>\n\n\n\n\n<p><a href=\"https:\/\/research.ncsu.edu\/cmif\/galleries\/image-gallery\/#autoheading-0\">See confocal workstation gallery page<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Laser Lines for Excitation<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>405 nm diode laser (for DAPI, ALexa 350)<\/li>\n\n\n\n<li>458 nm, 488 nm, 514 nm multiline argon laser (35 mW)<\/li>\n\n\n\n<li>561 nm diode pumped solid state laser<\/li>\n\n\n\n<li>633 nm HeNe laser<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Emission Windows<\/h2>\n\n\n\n<p>They can be flexibly selected with 2 flanking photomultiplier (pmt) channels and a 32 channel GaAsP detector array. The system can be opperated in lambda mode with up to 34 spectral detectors that collect the entire wavelength range in one scan or in channel mode with up to 10 simultaneously active detection channels that can be set as needed.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Differential Interference Contrast Images<\/h2>\n\n\n\n<p>Differential interference contrast images can be acquired simultaneously and DIC prisms are available for all objectives except 10x.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Airyscan<\/h2>\n\n\n\n<p>The Airyscan is a 32 channel area GaAsP detector that can be used to improve spatial resolution and signal to noise ratio.&nbsp; In super-resolution mode emission light through a pin hole (set at 1.25 AU) is spread over a 32-channel honeycomb detector, each channel simultaneously capturing a different position of the point spread function. After remapping and a deconvolution step the spatial resolution in x, y and z increases 1.7 fold. The S\/N improves 4-8 fold. The beamshaper for the fast airyscan mode uses 16 elements of the area detector and illuminates 4 points (in the y-axis) silmultaneously which speads up image aqucisition 4 fold. This comes at a small cost in resolution and S\/N compared to the regular airyscan mode but still has a 1.5x better resolution and 4x better S\/N than the regular confocal mode. Zeiss has produced in partnership with Bitesize webinars that give a detailed explanation on <a href=\"https:\/\/www.youtube.com\/watch?v=sGTNBXq8omg\" target=\"_blank\" rel=\"noopener\">how the airyscan works<\/a> and the <a href=\"http:\/\/bitesizebio.com\/webinar\/28696\/the-fast-module-for-zeiss-lsm-880-with-airyscan-confocal-super-resolution-imaging-with-four-times-the-speed-and-improved-signal-to-noise-ratio\/\" target=\"_blank\" rel=\"noopener\">advantages of the fast airyscan module.<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Microscope and Accessories<\/h2>\n\n\n\n<p>The scanhead is attached to a Zeiss Axio Observer Z1 inverted microscope with motorized, programmable x,y,z stage, additional piezo z-stage, joystick and touch panel docking control station. A definite focus (Version 2) attachment corrects for focus drifts during overnight time lapse experiments. Temperature and CO2 control chambers are available.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Objectives<\/h2>\n\n\n\n<table id=\"tablepress-19\" class=\"tablepress tablepress-id-19\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">Magnification<\/th><th class=\"column-2\">N.A. Immersion<\/th><th class=\"column-3\">Type<\/th><th class=\"column-4\">Notes<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping row-hover\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">10x<\/td><td class=\"column-2\">0.45 dry<\/td><td class=\"column-3\">Plan-Apochromat<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">20x<\/td><td class=\"column-2\">0.8 dry<\/td><td class=\"column-3\">Plan-Apochromat<\/td><td class=\"column-4\"><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">40x<\/td><td class=\"column-2\">1.1 W<\/td><td class=\"column-3\">C-Apochomat<\/td><td class=\"column-4\">long working distance<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">40x<\/td><td class=\"column-2\">1.2 W<\/td><td class=\"column-3\">C-Apochromat<\/td><td class=\"column-4\">airyscan<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">63x<\/td><td class=\"column-2\">1.4 oil<\/td><td class=\"column-3\">Plan-Apochromat<\/td><td class=\"column-4\">airyscan<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n<h2 class=\"wp-block-heading\">Additional Resources<\/h2>\n\n\n\n<p>Check out the Zeiss educational site to learn more about <a href=\"http:\/\/zeiss-campus.magnet.fsu.edu\/tutorials\/objectivecolorcoding\/index.html\" target=\"_blank\" rel=\"noopener\">objective specifications<\/a>. More information about the instrument can be found on the <a href=\"http:\/\/www.zeiss.com\/corporate\/en_us\/home.html\" target=\"_blank\" rel=\"noopener\">Zeiss webpage<\/a>.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"http:\/\/www.zeiss.com\/microscopy\/en_us\/products\/light-microscopes\/axio-observer-for-materials.html\" target=\"_blank\" rel=\"noopener\">Axio Observer<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Reservation and Rules<\/h2>\n\n\n\n<p>Once approved to use the Zeiss LSM 880 Airyscan confocal&nbsp;independently you can reserve&nbsp;time online by visiting the <a href=\"https:\/\/labs.ncsu.edu\" target=\"_blank\" rel=\"noopener\">NC State Lab Management <\/a>portal. Log in with your unity credentials. Navigate to CMIF and select Zeiss LSM 880. Be aware that the time allowed per appointment and number of appointments may be restricted when the resource is in high demand.&nbsp; Appointments can be canceled without charge 24 h before start time. If you need to cancel at shorter notice please send an email notification to&nbsp;CMIF. You may be charged for the time reserved during peak time (9am to 5pm).<\/p>\n\n\n\n<p>Please note: The last person signed up for the day is responsible for powering the system off. If you are the last person on the schedule and&nbsp;do not need to use the confocal you still have to come in to switch the system off or find someone else to do it. If you ask someone by email make sure you receive confirmation. Leaving the confocal on overnight will result in a $50 fine.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Please read the facility policy and contact CMIF to set up an appointment. The Zeiss LSM 880 with Airyscan is the latest and most advanced model in the Zeiss LSM&hellip;<\/p>\n","protected":false},"author":16,"featured_media":901,"parent":534,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"ncst_dynamicHeaderBlockName":"ncst\/default-header","ncst_dynamicHeaderData":"{}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-1851","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Zeiss LSM 880 confocal workstation with Airyscan - Cellular and Molecular Imaging Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.ncsu.edu\/cmif\/equipment-list\/workstations\/zeiss-lsm-880\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Zeiss LSM 880 confocal workstation with Airyscan - Cellular and Molecular Imaging Facility\" \/>\n<meta property=\"og:description\" content=\"Please read the facility policy and contact CMIF to set up an appointment. 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