{"id":2547,"date":"2023-02-22T16:58:32","date_gmt":"2023-02-22T21:58:32","guid":{"rendered":"https:\/\/research.ncsu.edu\/cmif\/?p=2547"},"modified":"2023-02-22T16:59:58","modified_gmt":"2023-02-22T21:59:58","slug":"new-advanced-microscope-workstations-are-available-at-our-centennial-campus-location-in-the-plant-science-building","status":"publish","type":"post","link":"https:\/\/research.ncsu.edu\/cmif\/new-advanced-microscope-workstations-are-available-at-our-centennial-campus-location-in-the-plant-science-building\/","title":{"rendered":"New advanced microscope workstations are available at our Centennial Campus location in the Plant Science Building"},"content":{"rendered":"\n<p>The new CMIF headquarters is officially open for all our current and future customers on Centennial Campus. The address is: 840 Oval Drive Raleigh NC, 27606, Suite 2170. This is an exciting expansion and a new milestone for CMIF. We will continue to support the Main Campus community and to upgrade the equipment in our \u201cold\u201d laboratory.&nbsp;<\/p>\n\n\n\n<p>Here is a list of state of the art microscopes that are hosted at our new location:<\/p>\n\n\n\n<p><strong>Olympus VS200 Slide Scanner<\/strong><\/p>\n\n\n\n<p>High throughput slide scanner for brightfield and fluorescence. Can accommodate six slides at a time. Color camera and ORCA Fusion b\/w sCMOS camera. Filter sets for DAPI, FITC, TRITC, Cy5 and Cy7. Wide range of objectives from 4x to 100x. Acquired through NCBC grant (PI: Troy Ghashghaei).<\/p>\n\n\n\n<p><strong>Leica Stellaris 8 confocal microscope with FALCON and LIGHTNING<\/strong><\/p>\n\n\n\n<p>Inverted microscope with x,y scanning stage and galvanometer z-stage, adaptive focus control, pulsed white light laser 440-790 nm and 405 nm diode laser; low noise HyD detectors with gating and photon counting, extended range detectors for Cy7, fast quantitative FLIM (FALCON), LIGHTNING superresolution, OKO stage top incubator. Acquired with CALS funds.<\/p>\n\n\n\n<p><strong>Zeiss LSM 980 with Airyscan 2 with Picoquant FLIM\/FRET<\/strong><\/p>\n\n\n\n<p>Inverted Axio Observer.7 with Definite Focus.3, full range of high end objectives with DIC, laser lines: 405, 445, 488, 514, 561, and 640 nm, 2PMTs + 4 GaAsp PMTs, scanning x,y stage, fast z-piezo stage, AI sample finder, automation via \u201ctile and position\u201d plug in, Airyscan Multiplex SR-8Y (8x faster than regular scanning has improved resolution und S\/N), live cell incubation. Picoquant addition: 4 pulsed lasers: 440\/483\/560\/640 nm, 2 detectors, PicoQuant Symphotime software. Picoquant system is superior system for quantitative FRET\/FLIM analysis of challenging samples with low signal. Acquired with CALS funds.<\/p>\n\n\n\n<p><strong>Leica LMD7 and Leica CM3050 cryotome<\/strong><\/p>\n\n\n\n<p>Leica LMD7 upright laser microdissection system with a dissection and collection unit, 8-fold fluorescence cube changer, Leica LED3 LMD fluo-cubes, Leica DFC7000 T Color camera, Leica DFC9000 GT Fluorescence Camera, 25x Plan fluotar for bright field (BF), 5x plan UVI microdissection for BF, 20x plan fluotar LWD for BF &amp; DIC, 40x plant fluotar XT LWD for BF &amp; DIC, 63x fluotar XT LWD for BF &amp; DIC, 100X HC fluotar for Bf &amp; DIC,3D deconvolution, 3D visualization and analysis. Can be used as a regular, upright, widefield fluorescence microscope. Acquired through USDA grant, lead PI: Justin Whitehill.<\/p>\n\n\n\n<p><strong>Leica Thunder for Model Organisms<\/strong><\/p>\n\n\n\n<p>High end dissecting microscope with scanning stage and multiple objectives (1x, 1.6x, 2x, and 5x); dedicated for brightfield or fluorescence imaging of larger specimens, with deconvolution and optical clearing (THUNDER technology). Leica DMC6200 color camera and Leica DFC9000 sCMOS b\/w camera. Acquired with CALS funds.<\/p>\n\n\n\n<p><strong>Leica S9<\/strong><\/p>\n\n\n\n<p>Stereo Microscope with color camera, used for prep work, no fluorescence and no motorized stage and focus. Brightfield, darkfield, polarized light, lower end color camera<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The new CMIF headquarters is officially open for all our current and future customers on Centennial Campus. The address is: 840 Oval Drive Raleigh NC, 27606, Suite 2170. This is&hellip;<\/p>\n","protected":false},"author":1024,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"ncst_dynamicHeaderBlockName":"","ncst_dynamicHeaderData":"","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"categories":[1],"tags":[],"class_list":["post-2547","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"displayCategory":null,"acf":{"ncst_posts_meta_modified_date":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>New advanced microscope workstations are available at our Centennial Campus location in the Plant Science Building - Cellular and Molecular Imaging Facility<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.ncsu.edu\/cmif\/new-advanced-microscope-workstations-are-available-at-our-centennial-campus-location-in-the-plant-science-building\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"New advanced microscope workstations are available at our Centennial Campus location in the Plant Science Building - Cellular and Molecular Imaging Facility\" \/>\n<meta property=\"og:description\" content=\"The new CMIF headquarters is officially open for all our current and future customers on Centennial Campus. 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