{"id":1005,"date":"2022-12-07T13:55:25","date_gmt":"2022-12-07T18:55:25","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=1005"},"modified":"2026-04-22T20:11:33","modified_gmt":"2026-04-23T00:11:33","slug":"other-genomic-services","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/genomic-services\/other-genomic-services\/","title":{"rendered":"Other Genomic Services"},"content":{"rendered":"\n\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Hi-Throughput-DNA-Isolation\">Hi-Throughput DNA Isolation<\/h2>\n\n\n\n<p><strong>LGC oKtopure<\/strong><\/p>\n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n      \n<div class=\"text-only wp-block-ncst-fp-accompaniment\">\n    \n\n<p>The oKtopure\u2122 is a fully automated nucleic acid extraction platform that combines high-throughput automation with the proprietary sbeadex\u2122 magnetic bead based extraction chemistry for high quality, high yield extraction.<br><br>Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads with the DNA in place while transferring the buffers required for washing, binding and elution steps. <\/p>\n\n\n  <\/div>\n\n\n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-square\">\n          <img \n        decoding=\"async\"\n        class=\"fp-image wp-image-\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.35.15-PM.png\"\n         alt=\"\"               >\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n    <\/div>\n  \n\n\n\n<p>The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping and NGS.<\/p>\n\n\n\n<p><strong>KingFisher Flex<\/strong><\/p>\n\n\n\n<p>The Thermo Scientific KingFisher Flex is a versatile benchtop automated extraction instrument in the lab for 24 or 96 samples per run. It offers consistent medium- to high-throughput extraction and purification of DNA, RNA, proteins, and cells.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"DNA\/RNA-Sample-QC\">DNA\/RNA Sample QC Services<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Available at Main GSL Facility (Thomas Hall)<\/strong><\/h3>\n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n      \n<div class=\"text-only wp-block-ncst-fp-accompaniment\">\n    \n\n<p><strong>Agilent 4200 Tapestation<\/strong><br><br>The GSL offers DNA sample QC on Agilent\u2019s newest TapeStation 4200 platform. This instrument performs size and integrity analysis (QC) and quantification of nucleic acids. Instead of chips, the TapeStation system uses credit card-sized, disposable Agilent ScreenTape available for RNA, DNA (up to 10kb) or High Molecular Weight DNA (up to 60 kb).<\/p>\n\n\n  <\/div>\n\n\n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-square\">\n          <img \n        decoding=\"async\"\n        class=\"fp-image wp-image-\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.38.14-PM.png\"\n         alt=\"\"               >\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n    <\/div>\n  \n\n\n\n<h3 class=\"wp-block-heading\">Available at Plant Genotyping Facility (PSB)<\/h3>\n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n      \n<div class=\"text-only wp-block-ncst-fp-accompaniment\">\n    \n\n<p><strong>Agilent 4200 Tapestation<\/strong><br><br>The Genotyping lab also operates an Agilent 4200 TapeStation, performing size\/integrity analysis and quantification of DNA samples. QC options are available for RNA, DNA (up to 10kb), and High Molecular Weight DNA (up to 60 kb).<\/p>\n\n\n  <\/div>\n\n\n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-square\">\n          <img \n        decoding=\"async\"\n        class=\"fp-image wp-image-\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.38.14-PM.png\"\n         alt=\"\"               >\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n    <\/div>\n  \n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n      \n<div class=\"text-only wp-block-ncst-fp-accompaniment\">\n    \n\n<p><strong>ThermoFisher Fluoroskan System<\/strong><br><br>The Genotyping lab utilizes a Thermo Fluoroskan fluorometric plate reader for high-throughput PicoGreen quantification of DNA samples.&nbsp;<br><br>Requests for sample QC services can be made through our&nbsp;<a href=\"https:\/\/research.ncsu.edu\/gsl\/how-to-submit-a-project\/\">Online Submission System<\/a><\/p>\n\n\n  <\/div>\n\n\n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-square\">\n          <img \n        decoding=\"async\"\n        class=\"fp-image wp-image-\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.40.19-PM.png\"\n         alt=\"\"               >\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n    <\/div>\n  \n\n\n\n<h2 class=\"wp-block-heading\" id=\"Blue-Pippin-Size-Selection\">BluePippin Size Selection<\/h2>\n\n\n\n<p><strong>Sage BluePippin&nbsp;<\/strong><\/p>\n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n      \n<div class=\"text-only wp-block-ncst-fp-accompaniment\">\n    \n\n<p>The BluePippin is an electrophoresis platform capable of size-selecting DNA fragments and automatically collects the fractions in buffer according to the user-specified size range. With up to 5 samples per run, it is commonly used to size-select next generation sequencing libraries. It is capable of performing narrow size selections from 50bp to 50kb within a few hours. Customers may drop off samples to be run by GSL personnel at&nbsp;<em>either<\/em>&nbsp;the Main GSL facility (Thomas Hall, Central Campus) or the Plant Genotyping Facility (Plant Sciences Building, Centennial Campus). Requests for this service are made through our&nbsp;<a href=\"https:\/\/research.ncsu.edu\/gsl\/how-to-submit-a-project\/\">Online Submission System<\/a>.<\/p>\n\n\n  <\/div>\n\n\n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-square\">\n          <img \n        decoding=\"async\"\n        class=\"fp-image wp-image-\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.49.32-PM.png\"\n         alt=\"\"               >\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n    <\/div>\n  \n","protected":false},"excerpt":{"rendered":"<p>Hi-Throughput DNA Isolation LGC oKtopure The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping and&hellip;<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":42,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"useCTA\":false,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageID\":54,\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-1005","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - 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