{"id":448,"date":"2022-08-10T10:20:30","date_gmt":"2022-08-10T14:20:30","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=448"},"modified":"2026-03-26T14:11:49","modified_gmt":"2026-03-26T18:11:49","slug":"faq","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/faq\/","title":{"rendered":"FAQ"},"content":{"rendered":"\n\n\n\n\n<h2 class=\"wp-block-heading\" id=\"general\">General<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-e04mgo\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\">Where are you located?<\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>The <strong>main GSL facility<\/strong> is located on the second floor (room 2518) of Thomas Hall on the North Campus of NC State University. The GSL <strong>Plant Genotyping Facility<\/strong> is located on the second floor (rooms 2364 and 2368) of the Plant Sciences Building on Centennial Campus. For maps and contact information, visit&nbsp;<a href=\"http:\/\/research.ncsu.edu\/gsl\/contact-us\/\">Contact Us<\/a>.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>How do I submit a project to you and what forms do I need to fill out?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>The GSL uses an online Lab Submission system for projects. For more information on access, as well as a user\u2019s guide, please see our <a href=\"https:\/\/research.ncsu.edu\/gsl\/how-to-submit-a-project\/\" data-type=\"page\" data-id=\"128\">How To Submit Projects<\/a> page.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>What time can I stop by to drop off samples?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Our hours for sample drop-off are 9 AM until 4 PM Monday through Friday. We ask that if you want to stop by during lunch (12-1 PM) that you call ahead to confirm your plans (919-513-3882).<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Can I schedule a meeting to discuss a project I had in mind? How do I get a quote?<\/strong><br><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Of course! We encourage you to discuss your sequencing project needs with us. Please email or call Kelly (919-515-2012; <a href=\"mailto:kafridey@ncsu.edu\">kafridey@ncsu.edu<\/a>) to set-up a time.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you accept projects from outside of NC State University or outside of the United States?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Yes. We have many customers from all over the country and around the world. Please call or email Kelly (919-515-2012; <a href=\"mailto:kafridey@ncsu.edu\">kafridey@ncsu.edu<\/a>) for questions about billing concerns.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Are your prices different for NC State customers, other universities, or for businesses?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>All academic or U.S. federally-funded parties are currently charged the same use rates. Other parties or businesses, please <a href=\"https:\/\/research.ncsu.edu\/gsl\/contact\/\" data-type=\"page\" data-id=\"606\">Contact Us<\/a> to discuss pricing.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"e04mgo\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>Do you offer downstream bioinformatics analysis services?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>We are strictly a wet lab and unfortunately do not offer bioinformatic services. Our endpoint deliverables are demultiplexed .fastq files (Illumina) or .bam files (PacBio). Please contact the NC State Bioinformatics Consulting and Service Core (<a href=\"mailto:genomicsci-support@ncsu.edu\">genomicsci-support@ncsu.edu<\/a>) to schedule a consultation to discuss your research needs after receiving your data.&nbsp;<\/p>\n\n\n\n<p>We do offer a draft contig assembly for our Phage Sequencing Service using CLC Genomics Workbench.&nbsp;<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Sanger-DNA-Sequencing\">Sanger DNA Sequencing<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-khzwxm\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"khzwxm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What concentration does my PCR product need to be for Sanger (Capillary) DNA Sequencing?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Please see our&nbsp;<a href=\"http:\/\/research.ncsu.edu\/gsl\/sequencing\/sanger-sequencing\/#autoheading-1\">Sanger Sequencing Guidelines<\/a>&nbsp;for specific details.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"khzwxm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you add primers for Sanger DNA Sequencing?<\/strong><br><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>For <em>Gold service<\/em>, we typically require the user to add specific primers to their DNA template, for each reaction. For <em>Platinum service<\/em>, as we perform Exosap cleanup of the PCR template, we therefore request users provide their primer in a separate tube which will be added following the ExoSap step. More information about these specifications can be found on our <a href=\"https:\/\/research.ncsu.edu\/gsl\/sanger-sequencing\/sanger-sequencing-guidelines\/\" data-type=\"page\" data-id=\"108\">Sanger DNA submission guidelines<\/a> page.<\/p>\n\n\n\n<p>The GSL <em>does<\/em> maintain stocks of common vector-based sequencing primers, in which users can request to be added (either Gold or Platinum service). These primers are M13F, M13R, T7, or Sp6.<\/p>\n\n\n\n<p>M13F(- 41): 5&#8242; &#8211; CGC CAG GGT TTT CCC AGT CAC GAC &#8211; 3&#8242;<br>M13R(- 48): 5&#8242; &#8211; AGC GGA TAA CAA TTT CAC ACA GG &#8211; 3&#8242;<br>T7 Promoter: 5&#8242; &#8211; TAA TAC GAC TCA CTA TAG GG &#8211; 3&#8242;<br>SP6 Promoter: 5&#8242; &#8211; TAC GAT TTA GGT GAC ACT ATA G &#8211; 3&#8242;<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"khzwxm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What size tubes should I submit my samples in?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>For <em>Sanger DNA submissions<\/em>, <strong>for 8 or less samples<\/strong>, users may submit their samples in clearly labeled 1.5 mL microcentrifuge tubes or PCR strip tubes. <strong>For more than 8 samples<\/strong>, please submit your samples in a 96 well plate (even if requesting &lt; 96 reactions) or use 8-strip pcr tubes\/caps. Users submitting samples by FedEx\/UPS delivery should ensure the samples are adequately sealed and protected during shipping.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"khzwxm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What time must I drop-off my samples if I wish same-day service?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>**Please note only Gold and Bronze Sanger Sequencing services are available for next day service**&nbsp;<\/p>\n\n\n\n<p>For same day data delivery, we <em>must have your <a href=\"https:\/\/labs.ncsu.edu\">Lab Submission Order<\/a> no later than 9:30 AM<\/em> on the day of service, and <em>samples must arrive at the GSL by 10:30 AM<\/em> that same day. Orders\/samples received after these times will be delivered the following business day. Users requesting our on campus courier service will need to have their samples in the courier dropbox <em>no later than 10:00 AM<\/em>.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"khzwxm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>When will I get my Sanger results?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>For Gold and Bronze services, if you meet the time criteria for Order submission and sample drop off (see above FAQ), <em>you should have results sent by email by around 5 PM the same day<\/em>. If you were not able to meet this time criteria, or are submitting samples for Platinum sequencing, you should get results by 5 PM the next business day.<\/p>\n\n\n\n<p><em>Note:<\/em> The GSL will make every effort to adhere to this regimen, however there are times when data delivery may be delayed due to factors beyond our control (instrumentation issues, supply backorders, or a higher than expected submission volume). <em>We cannot fully guarantee same-day delivery for your samples.<\/em><\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n\n\n<div class=\"has-custombg-five-background-color wp-block-ncst-button-spotlight\">\n    <div class=\"button-spotlight__container\">\n          <div class=\"button-spotlight__icon-container ncst-icon\">\n        <svg viewBox=\"0 0 24 24\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<path d=\"M23.9999 1.9664H22.2463V22.0336H23.9999V1.9664Z\" fill=\"currentColor\"\/>\n<path d=\"M1.30219 9.89015H0V14.1094H1.30219V9.89015Z\" fill=\"currentColor\"\/>\n<path d=\"M21.4787 4.19952L2.07056 10.5141V13.4859L6.38306 14.8889C6.35454 14.9524 6.32966 15.0175 6.30853 15.0839C6.15766 15.5612 6.20229 16.0789 6.43261 16.5234C6.66293 16.9679 7.06014 17.3028 7.53712 17.4548L9.77962 18.1664C10.257 18.3166 10.7743 18.2716 11.2186 18.0412C11.6628 17.8109 11.9978 17.414 12.1501 16.9373C12.167 16.8848 12.1801 16.8319 12.1918 16.7789L21.4782 19.8005L21.4787 4.19952ZM11.2656 16.6566C11.1876 16.8984 11.0173 17.0997 10.7917 17.2167C10.5661 17.3336 10.3034 17.3567 10.0609 17.2809L7.81837 16.5698C7.57594 16.4925 7.37407 16.3223 7.25702 16.0963C7.13997 15.8704 7.11731 15.6073 7.19399 15.3647C7.21538 15.3004 7.24334 15.2385 7.27743 15.18L11.3007 16.4887C11.2942 16.5457 11.2825 16.6018 11.2656 16.6566Z\" fill=\"currentColor\"\/>\n<\/svg>\n\n      <\/div>\n        <div class='button-spotlight__content-container'>\n      <h2 class=\"button-spotlight__heading\">Sunsetting Select Services<\/h2>\n              <p class=\"button-spotlight__support\">Effective Feb. 2, 2026, the GSL will no longer offer Sanger sequencing or fragment genotyping, as part of a broader effort to streamline our services.<\/p>\n            \n<div class=\"is-style-secondary wp-block-ncst-button\">\n      <a\n        class=\"ncst-block__button-link btn\"\n        href=\"https:\/\/research.ncsu.edu\/gsl\/dna-sequencing\/sanger-sequencing-fragment-genotyping\/sunsetting-faq\/\"\n        data-ncst-lightbox=\"false\"\n                      >\n                  <span class=\"text\">Learn more<\/span><span class=\"arrow-indicator\"><svg class=\"wolficon wolficon-arrow-right-bold\" role=\"img\"  aria-hidden=\"true\">\n\t\t\t\n\t\t\t<use xlink:href=\"#wolficon-arrow-right-bold\">\n\t\t<\/svg><\/span>\n              <\/a>\n    <\/div>\n  \n\n    <\/div>\n  <\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Next-Generation-Sequencing\">Next Generation Sequencing<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-3sc7cn\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"3sc7cn\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What kind of NGS library preps do you offer?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Please see our NGS Library Prep page for more information on the types of NGS library preparation services we offer.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"3sc7cn\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>How much DNA\/RNA do I need to submit for Next Generation Sequencing Library preps?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Although it depends on the specific type of NGS library prep you are interested in, a good starting point is 1-2 \u03bcg of HMW DNA or RNA in a volume of 40-50 \u03bcl (20-50 ng\/\u03bcl). If you are unable to prepare this amount, or need more information on specific library prep requirements, please contact the <a href=\"mailto:gsl_support@ncsu.edu\">GSL team<\/a>.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"3sc7cn\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you accept user-prepared NGS libraries for sequencing?<\/strong><br><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>We do accept user prepared libraries for sequencing, under the following criteria.<\/p>\n\n\n\n<p>For <em>Illumina libraries<\/em>, we require a final pooled library at 10 nM (we do not offer pooling services). For each lane or flow cell requested, please provide a volume of 30 \u03bcL. <em>If your library is significantly lower than 10nM<\/em>, please <a href=\"https:\/\/research.ncsu.edu\/gsl\/contact\/\" data-type=\"page\" data-id=\"606\">contact us<\/a> to see if it can be sequenced. <em>Note:<\/em> if requesting a full NovaSeq S4 flow cell, please submit a volume of 120 \u03bcL (4 XP lanes per flow cell).<\/p>\n\n\n\n<p>For <em>PacBio libraries<\/em>, please provide a final library pool at 20 ng\/\u03bcL with at least a 10 \u03bcL volume.<\/p>\n\n\n\n<p>** Disclaimer 1** &#8211; the GSL adds a mandatory QC charge of $32.00, which is necessary for proper loading. While we will report any issues we see with the library QC spectra to customers before sequencing, the GSL <em>cannot guarantee results for libraries we do not make<\/em>, and will need to charge regardless of the outcome.<\/p>\n\n\n\n<p>** Disclaimer 2** &#8211; for <em>single XP lane options<\/em> (Illumina NovaSeq 6000), customer libraries must allow standard Illumina index and read cycling settings, with no custom primer addition, as these impact other lanes on the flow cell. Whole flow cell selections do not require this. Please <a href=\"https:\/\/research.ncsu.edu\/gsl\/contact\/\" data-type=\"page\" data-id=\"606\">Contact Us<\/a> for more information.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"3sc7cn\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What type of Next Generation Sequencing services do you offer?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>We currently house two Illumina MiSeq sequencers, one NextSeq 2000 sequencer, one NovaSeq 6000 sequencer, and one PacBio Sequel IIe in our facilities. Please see our <a data-type=\"page\" data-id=\"179\" href=\"https:\/\/research.ncsu.edu\/gsl\/ngs-platforms\/\">NGS Platforms<\/a> page for more information on these types of NGS sequencers.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"3sc7cn\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>How do you recommend shipping RNA or DNA to your lab?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>We recommend shipping DNA overnight with freezer packs. For RNA we recommend shipping on dry ice, with the total RNA in nuclease-free water (50 \u03bcl). If the sample is mRNA, please contact the GSL to determine the appropriate volume.<\/p>\n\n\n\n<p>Please see our <a href=\"https:\/\/research.ncsu.edu\/gsl\/contact\/\" data-type=\"page\" data-id=\"606\">Contact Us<\/a> page for facility shipping information.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"DNA-&amp;-RNA-Extractions\">DNA and RNA Extractions<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-6ah2am\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"6ah2am\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you offer DNA extraction as a service<\/strong>?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>The GSL offers high-throughput DNA extraction services using either the LGC Oktopure or Thermo Kingfisher robotics platforms. For more information, please visit our HT DNA isolation page, as well as our DNA extraction guidelines.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"6ah2am\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you extract RNA for customers?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>The GSL does offer RNA extractions <em>under certain conditions<\/em>. Please see our RNA extraction guidelines for more information on this service.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Bioanalyzer,-Tapestation-&amp;-BluePippin\">Bioanalyzer, Tapestation and BluePippin<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-vvmhcf\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you offer DNA\/RNA sample QC services?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>The GSL offers a variety of \u00e0 la carte QC services for DNA and RNA. Please visit our DNA\/RNA Sample QC page for more information.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do I need to submit a separate Bioanalyzer\/Tapestation request form if I am also requesting NGS library prep and sequencing?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p><em>No.<\/em> For NGS <a data-type=\"page\" data-id=\"128\" href=\"https:\/\/research.ncsu.edu\/gsl\/how-to-submit-a-project\/\">project submissions<\/a>, Bioanalyzer and Tapestation QC components are included directly in the NGS form. <em>Users should only fill out a Bioanalyzer\/Tapestation request form if they just want QC services <\/em>(for example, if you are testing different isolation protocols or making your own NGS libraries). <em>The GSL only accepts small, disposable sample aliquots for this service<\/em>.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>What concentration range should my samples be for RNA QC (Bioanalyzer)?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>For the RNA Pico chip, please bring your samples at a concentration of 1-5 ng\/\u03bcL. For the RNA Nano chip, samples may be at 50-200 ng\/\u03bcL.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>What concentration\/size range should my samples be for DNA QC (Tapestation)?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>We offer five different Tapestation assays. For HMW genomic DNA (100 bp to 60 kb) please bring your samples at a concentration of 10-100 ng\/uL. If you are interested in fragments between 100 and 1000 bp, samples should be at 0.1 &#8211; 50 ng\/uL for the D1000 assay or 10-1000 pg\/uL for the High Sensitivity D1000.&nbsp; If you are interested in fragments between 100 and 5000 bp, samples should be at 0.1 &#8211; 50 ng\/uL for the D5000 assay or 10-1000 pg\/uL for the High Sensitivity D5000.&nbsp;<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>How much sample do I need to bring?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Please bring 3-5 \u03bcl of each sample, at the appropriate concentration, in 1.7 mL tubes, labeled clearly. For picogreen or HT services, please submit your samples in a 96w plate, arranged <em>by column<\/em> (not rows).<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"vvmhcf\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong>How do I prepare my samples for BluePippin size selection?<\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>BluePippin samples should be submitted in exactly 30 \u03bcl of EB, TE, or water. For more information about this service, please visit our BluePippin page.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Other-Questions\">Other Questions<\/h2>\n\n\n<div class=\"is-text wp-block-ncst-accordion\" >\n    <div class=\"accordion isLinked\" id=\"ncst-accordion-828fhm\">\n          \n<details class=\"wp-block-ncst-accordion-item\" name=\"828fhm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong><strong>Do you let customers use your lab equipment?<\/strong><\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>While many of our instrumentation can be used only by GSL staff, we do operate and maintain shared user equipment at both our main GSL facility and the Plant Genotyping facility that is available for use after training. Please see our Shared User Equipment section for more information.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"828fhm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong><strong>How do I request shared instrument access and get trained?<\/strong><\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p><em>Training requests and use scheduling are made through the GSL <a href=\"https:\/\/labs.ncsu.edu\">Lab Submission<\/a> system. For a user\u2019s guide to using this system, please see our <a href=\"https:\/\/research.ncsu.edu\/gsl\/how-to-submit-a-project\/\" data-type=\"page\" data-id=\"128\">How to Submit Projects<\/a> page.<\/em><\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"828fhm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong><strong>Do you offer qPCR services, or set up qRT-PCR plates?<\/strong><\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>No. We offer qPCR instrumentation use as shared equipment, and our customers set up their own assay plates and run them. Training requests and use scheduling are made through the GSL <a href=\"https:\/\/labs.ncsu.edu\" data-type=\"URL\" data-id=\"https:\/\/labs.ncsu.edu\">Lab Submission<\/a> system.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n<details class=\"wp-block-ncst-accordion-item\" name=\"828fhm\"  >\n      <summary class=\"accordion-item__header\">\n        <span class=\"accordion-item__expansion-indicator\">\n          <span class=\"ncst-plus-minus-toggle\"><\/span>\n        <\/span>\n        <h2 class=\"accordion-item__label h6\"><strong><strong>Do you train customers on how to prepare NGS libraries?<\/strong><\/strong><\/h2>\n      <\/summary>\n      <p>\n        \n\n<p>Currently we cannot offer direct in person training due to our work volume. We can provide customers with a review of relevant protocols, and further Q&amp;A. Additional opportunities for NGS library prep training, as well as other applications, may be available periodically through the NC State University <a href=\"https:\/\/biotech.ncsu.edu\/\">Biotechnology<\/a> program.<\/p>\n\n\n      <\/p>\n  <\/details>\n\n\n    <\/div>\n  <\/div>\n","protected":false},"excerpt":{"rendered":"<p>General Sanger DNA Sequencing Next Generation Sequencing DNA and RNA Extractions Bioanalyzer, Tapestation and BluePippin Other Questions<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"useCTA\":false,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageID\":54,\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-448","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - 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