{"id":448,"date":"2022-08-10T10:20:30","date_gmt":"2022-08-10T14:20:30","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=448"},"modified":"2026-03-26T14:11:49","modified_gmt":"2026-03-26T18:11:49","slug":"faq","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/faq\/","title":{"rendered":"FAQ"},"content":{"rendered":"\n\n\n\n\n
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The main GSL facility<\/strong> is located on the second floor (room 2518) of Thomas Hall on the North Campus of NC State University. The GSL Plant Genotyping Facility<\/strong> is located on the second floor (rooms 2364 and 2368) of the Plant Sciences Building on Centennial Campus. For maps and contact information, visit Contact Us<\/a>.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n The GSL uses an online Lab Submission system for projects. For more information on access, as well as a user\u2019s guide, please see our How To Submit Projects<\/a> page.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n Our hours for sample drop-off are 9 AM until 4 PM Monday through Friday. We ask that if you want to stop by during lunch (12-1 PM) that you call ahead to confirm your plans (919-513-3882).<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n Of course! We encourage you to discuss your sequencing project needs with us. Please email or call Kelly (919-515-2012; kafridey@ncsu.edu<\/a>) to set-up a time.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n Yes. We have many customers from all over the country and around the world. Please call or email Kelly (919-515-2012; kafridey@ncsu.edu<\/a>) for questions about billing concerns.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n All academic or U.S. federally-funded parties are currently charged the same use rates. Other parties or businesses, please Contact Us<\/a> to discuss pricing.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n We are strictly a wet lab and unfortunately do not offer bioinformatic services. Our endpoint deliverables are demultiplexed .fastq files (Illumina) or .bam files (PacBio). Please contact the NC State Bioinformatics Consulting and Service Core (genomicsci-support@ncsu.edu<\/a>) to schedule a consultation to discuss your research needs after receiving your data. <\/p>\n\n\n\n We do offer a draft contig assembly for our Phage Sequencing Service using CLC Genomics Workbench. <\/p>\n\n\n <\/p>\n <\/details>\n\n\n <\/div>\n <\/div>\n\n\n\n \n \n\n Please see our Sanger Sequencing Guidelines<\/a> for specific details.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n For Gold service<\/em>, we typically require the user to add specific primers to their DNA template, for each reaction. For Platinum service<\/em>, as we perform Exosap cleanup of the PCR template, we therefore request users provide their primer in a separate tube which will be added following the ExoSap step. More information about these specifications can be found on our Sanger DNA submission guidelines<\/a> page.<\/p>\n\n\n\n The GSL does<\/em> maintain stocks of common vector-based sequencing primers, in which users can request to be added (either Gold or Platinum service). These primers are M13F, M13R, T7, or Sp6.<\/p>\n\n\n\n M13F(- 41): 5′ – CGC CAG GGT TTT CCC AGT CAC GAC – 3′ \n \n\n For Sanger DNA submissions<\/em>, for 8 or less samples<\/strong>, users may submit their samples in clearly labeled 1.5 mL microcentrifuge tubes or PCR strip tubes. For more than 8 samples<\/strong>, please submit your samples in a 96 well plate (even if requesting < 96 reactions) or use 8-strip pcr tubes\/caps. Users submitting samples by FedEx\/UPS delivery should ensure the samples are adequately sealed and protected during shipping.<\/p>\n\n\n <\/p>\n <\/details>\n\n\n \n \n\n **Please note only Gold and Bronze Sanger Sequencing services are available for next day service** <\/p>\n\n\n\n\n \n <\/span>\n <\/span>\n
How do I submit a project to you and what forms do I need to fill out?<\/strong><\/h2>\n <\/summary>\n
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What time can I stop by to drop off samples?<\/strong><\/h2>\n <\/summary>\n
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Can I schedule a meeting to discuss a project I had in mind? How do I get a quote?<\/strong>
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Do you accept projects from outside of NC State University or outside of the United States?<\/strong><\/strong><\/h2>\n <\/summary>\n
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Are your prices different for NC State customers, other universities, or for businesses?<\/strong><\/strong><\/h2>\n <\/summary>\n
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Do you offer downstream bioinformatics analysis services?<\/strong><\/h2>\n <\/summary>\n
Sanger DNA Sequencing<\/h2>\n\n\n
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What concentration does my PCR product need to be for Sanger (Capillary) DNA Sequencing?<\/strong><\/strong><\/h2>\n <\/summary>\n
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Do you add primers for Sanger DNA Sequencing?<\/strong>
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M13R(- 48): 5′ – AGC GGA TAA CAA TTT CAC ACA GG – 3′
T7 Promoter: 5′ – TAA TAC GAC TCA CTA TAG GG – 3′
SP6 Promoter: 5′ – TAC GAT TTA GGT GAC ACT ATA G – 3′<\/p>\n\n\n <\/p>\n <\/details>\n\n\n\n \n <\/span>\n <\/span>\n
What size tubes should I submit my samples in?<\/strong><\/strong><\/h2>\n <\/summary>\n
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What time must I drop-off my samples if I wish same-day service?<\/strong><\/strong><\/h2>\n <\/summary>\n