{"id":499,"date":"2022-08-10T11:22:10","date_gmt":"2022-08-10T15:22:10","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=499"},"modified":"2025-06-13T13:47:15","modified_gmt":"2025-06-13T17:47:15","slug":"sponsored-projects","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/news\/sponsored-projects\/","title":{"rendered":"GSL Sponsored Seminars & Events"},"content":{"rendered":"\n\n\n\n\n
\n Sponsored by the NC State Genetics & Genomics Initiative<\/a>, the Center for Human Health and the Environment<\/a>, and the NC State Genomic Sciences Laboratory<\/a>.<\/strong>\n <\/p>\n \n 9:30 \u2013 10:15 AM:<\/strong> Installing CLC, connecting to your network, how to connect to the HPC, Introduction to Qiagen Bioinformatic Solutions<\/p>\n\n\n\n 10:30 \u2013 12:00 PM:<\/strong> RNA-seq data analysis<\/p>\n\n\n\n 12:00-1:00 PM: LUNCH <\/strong>** Lunch will be provided to registered participants **<\/p>\n\n\n\n 1:00-2:15 PM: <\/strong>Microbiome analysis: 16S\/shotgun sequencing<\/p>\n\n\n\n 2:30-3:30 PM: <\/strong>Genome and Metagenome assembly<\/p>\n\n\n\n See Event Flyer<\/a> for more information.<\/p>\n\n\n See Event Flyer<\/a> for more information.<\/p>\n\n\n Please join us for a hands on experience of our easy and efficient, Nextera DNA Flex Library Prep workflow for WGS of any genome, using your own samples on the GSL NovaSeq 6000. Learn from expert Illumina scientists as they guide you through: Library Preparation & Best Practices, Experimental Design for DNA sequencing Projects, and Data Analysis in BaseSpace\u2122Sequence Hub. Sequencing data will be delivered by the Genomic Sciences Laboratory, followed by a review Q&A session with the Illumina workshop team.<\/p>\n\n\n Presented by Nick Beckloff, Ph.D., FAS<\/p>\n\n\n\n Hosted by the NC State Genetics & Genomics Initiative and the Genomic Sciences Laboratory. See Event Flye<\/a>r<\/a> for more information.<\/p>\n\n\n Please join us for a hands on experience of our easy and efficient, Nextera DNA Flex Library Prep workflow for WGS of any genome, using your own samples on the GSL NovaSeq 6000. Learn from expert Illumina scientists as they guide you through: Library Preparation & Best Practices, Experimental Design for DNA sequencing Projects, and Data Analysis in BaseSpace\u2122Sequence Hub. Sequencing data will be delivered by the Genomic Sciences Laboratory, followed by a review Q&A session with the Illumina workshop team.<\/p>\n\n\n Chromosome conformation capture methods like Hi-C measure the 3D organization of DNA in vivo<\/em> using a combination of crosslinking, proximity-ligation, and paired-end sequencing.<\/p>\n\n\n\n Because this method captures genomic contiguity on intact chromosomes, the resultant information can be used to generate end-to-end chromosome-scale scaffolds for large genomes. Since Hi-C junctions form within intact cells, any sequences interacting by Hi-C must have originated from the same species\/strain in a mixed population, enabling metagenomic deconvolution.<\/p>\n\n\n\n 9:00 \u2013 9:45 AM:<\/strong> Installing CLC, connecting to your network, how to connect to the HPC, network rules\/best practices, data transfer, GSL website tour.<\/p>\n\n\n\n 9:45 \u2013 10:30 AM:<\/strong> Introduction: Why Genomics Workbench?: Genomics Workbench user interface and concept of workflow.<\/p>\n\n\n\n 10:30 \u2013 12:00 PM:<\/strong> DNA sequencing and Variant calling<\/p>\n\n\n\n 12:00-1:00 PM: LUNCH ** Lunch will be provided to registered participants **<\/p>\n\n\n\n 1:00-2:00 PM: <\/strong>RNAseq analysis<\/p>\n\n\n\n 2:00-3:30 PM: <\/strong>Microbial Genomics Module (Plugin) \u2013 Introduction and Taxonomic Profiling (Amplicon-based vs Whole Genome).<\/p>\n\n\n\n 3:30-4:30 PM:<\/strong> De novo<\/em> sequencing and Genome Finishing (Plugin) Overview<\/p>\n\n\n\n 4:30-5:00 PM:<\/strong> Flex time, other topics of interest.<\/p>\n\n\n SPEAKER: John Chuckalovcak, Senior Field Application Scientist, Bio-Rad Laboratories<\/p>\n\n\n\n Whether you are a first-time qPCR user or an experienced researcher trying to decide if digital PCR is right for you, this course will provide you with the knowledge and skill to design the right experiment and extract the most meaningful and publishable results from your data. What you will learn: Real-Time PCR<\/strong> \u2013 Best Practices and Potential Pitfalls (and how to avoid them), Bio-Rad\u2019s statistical analysis software<\/strong>: How to get the most out of your qPCR data; Droplet Digital PCR<\/strong> \u2013 How it works and when to use it.<\/p>\n\n\n\n Seminar Agenda: \u201cSMRT Sequencing Technology and Application Overview\u201d by Jay Morris, PacBio Field Application Scientist, followed by \u201cGetting Started with SMRT Sequencing Analysis\u201d by Kristina Weber, Bioinformatics Field Support. Then, one of our NCSU faculty, Dr. Marce Lorenzen will present some her PacBio research, entitled \u201cYou can take the high road AND the low road: Experiments that benefit from different levels of PacBio coverage.\u201d Attend the seminar for more information on a local PacBio SMRT grant opportunity.<\/p>\n\n\n\n Better Targeted & Exome Sequencing<\/strong> \u2013 Join us for a lunch and learn about exome and targeted sequencing. We\u2019ll cover how Twist\u2019s silicone-based DNA synthesis platform has revolutionized target enrichment, allowing researchers to sequence less and discover more. We\u2019ll discuss our double-stranded probes, GC-boosted designs, and easy-to-design custom panels that have a market-leading three week turn-around-time. Hosted by the Genomics Sciences Laboratory, lunch provided by Twist. Speaker: Mark Consugar \u2013 TWIST Field Application Scientist.<\/p>\n\n\n\n Metabolon<\/a> is the global leader in metabolomics and has developed an unbiased global approach to profiling the entire innate human metabolome and a vast amount of xenobiotics. Metabolomics provides proximity to disease, as small molecules are the closest molecular trait to the observed phenotype that represents the functions of the genes in the environment at that particular time. Unlike other \u2018omics\u2019 technologies, metabolomics provides a biochemical signature that takes into account not only genetics, but also the effects of lifestyle, diet and the environment on the health status of an individual.<\/p>\n\n\n\n Chromosome conformation capture methods like Hi-C measure the 3D organization of DNA in vivo using a combination of crosslinking, proximity-ligation, and paired-end sequencing. Because this method captures genomic contiguity on intact chromosomes, the resultant information can be used to generate end-to-end chromosome-scale scaffolds for large genomes. Since Hi-C junctions form within intact cells, any sequences interacting by Hi-C must have originated from the same species\/strain in a mixed population, enabling metagenomic deconvolution. Capturing genomic proximity information in vivo removes several major obstacles in genome and metagenome assembly, improving the quality and efficiency of genomic discovery efforts.<\/p>\n\n\n\n A contiguous and accurate genome assembly is a crucial first step in fully understanding the biology of any organism. A high-quality genome assembly will make any downstream analyses, like gene annotation, synteny, comparative genomics and population genetics far easier and more reliable. Dovetail Genomics is the leading service provider for high quality genome assemblies. To date, we have completed more than 400 projects, spanning many classes of organisms from plants to reptiles, amphibians, fish, mammals, birds, insects and more. Using our two complementary scaffolding methods, Chicago and Dovetail Hi-C, we are dramatically increasing the contiguity and accuracy of genome assemblies, enabling true, full-chromosome-length scaffolding. This talk will provide an update on our most current technologies. We will profile several customer projects and discuss how improvements in their assemblies led to better science and new discoveries.<\/p>\n\n\n CRISPR-Cas9 systems provide a platform for high- efficiency genome editing that can lead to innovative applications in cell engineering. However, the delivery of Cas9 and the synthesis of guide RNA (gRNA) remain as the two steps that can limit overall efficiency and general ease of use. In the technical seminar, \u201cHow to boost your CRISPR editing efficiencies,\u201d we\u2019ll discuss the rate-limiting steps in current CRISPR-Cas9 workflows, and introduce streamlined methods to help reduce the editing workflow timeline to just 4 days.<\/p>\n\n\n\n Guest Speaker: Dr. Paul Chomet<\/p>\n\n\n\n NGS technologies have opened the door to multiple genome analyses and an increased understanding of the variations present in populations. To date, most of the germplasm analyses have relied on the comparison of sequence reads to one reference genome assembly, limiting our understanding of genomic variation. NRGene has developed novel analytics and approaches to efficiently de- novo assemble genomes and describe the relevant variation across germplasm using a pan-genome approach. Using this approach, we are able to perform critical analyses including, haplotype imputation from sequence based genotyping and marker selection for array design to aid in molecular breeding and gene discovery.<\/p>\n\n\n\n 8:00-10:00 AM:<\/strong> Installing CLC, connecting to your network, how to connect to the HPC, network rules\/best practices, data transfer, GSL website navigation.<\/p>\n\n\n\n 10:15-11:00 AM:<\/strong> Basic software overview and intro to Biomedical WB, intro to the user interface, \uf0e0importing NGS data,reference management, and intro to the workflow concept and overview of all the tools.<\/p>\n\n\n\n 11:00-12:00 PM:<\/strong> Create workflows for read mapping, variant calling (with Bisulfite Sequencing focus)<\/p>\n\n\n\n 12:00-1:00 PM:<\/strong> LUNCH ** Lunch will be provided to all registered participants **<\/p>\n\n\n\n 1:00-2:00 PM \u2013 Workshop 1:<\/strong> Review results from Bisulfite sequencing and working with Tracks<\/p>\n\n\n\n 2:00-3:30 PM \u2013 Workshop 2:<\/strong> RNAseq analysis<\/p>\n\n\n\n 3:45-5:00 PM \u2013 Workshop 3:<\/strong> CLC Genomics to Ingenuity Pathway Analysis (Advanced)<\/p>\n\n\n\n 8:00-10:00 AM:<\/strong> Installing CLC, connecting to your network, how to connect to the HPC, network rules\/best practices, data transfer, GSL website tour.<\/p>\n\n\n\n 10:15-11:00 AM:<\/strong> Basic software overview, intro to the user interface\uf0e0, importing NGS data, downloading from SRA, downloading from NCBI, intro to the workflow concept and overview of all the tools.<\/p>\n\n\n\n 11:00-12:00 PM:<\/strong> Create workflows for de novo<\/em> assembly<\/p>\n\n\n\n 12:00-1:00 PM: LUNCH ** Lunch will be provided to all registered participants **<\/p>\n\n\n\n 1:00-2:00 PM \u2013 Workshop 1:<\/strong> De novo<\/em> assembly (Illumina and PacBio)<\/p>\n\n\n\n 2:00-3:00 PM \u2013 Workshop 2:<\/strong> Genome Finishing Analysis (Plugin)<\/p>\n\n\n\n 3:00-4:00 PM \u2013 Workshop 3:<\/strong> Microbial Genomics Analysis (Plugin)<\/p>\n\n\n\n 4:00-5:00 PM \u2013 Workshop 4:<\/strong> Advanced RNAseq analysis<\/p>\n\n\n\n Powerful molecular biology tools and methods are common. However, data produced by these tools and assays typically show the average signals from a bulk sample made up of many cells. In fields such as hematology, cancer biology, and cell biology, these methods may not yield the proper data, because the cells of interest may comprise only a small minority in a heterogeneous sample, hence their behavior is drowned out by that of the majority. Accurate characterization of these minority cells can only be performed by single cell analysis. In this presentation, I will explain BD\u2019s new technologies for single cell analysis, and how they may be combined with FACS to produce data that links phenotypic markers to genotypic expression<\/p>\n\n\n\n Learn about best practices for laboratory setup when working with RNA, and how to select the right isolation kit for your sample type. Choose between qPCR, next-gen sequencing, or microarray workflows to interrogate your samples as you study gene expression, the transcriptome, small RNA\u2019s, splice variants, and fusions. We will also cover the basics of the data output from each of these platforms and how you can analyze it. Finally, compare the strengths of each to determine which platform is best suited to your project needs<\/p>\n\n\n\n Please join us for a next-generation sequencing (NGS) seminar!Illumina sequencing and genotyping technologies touch many steps of the agrigenomics pipeline. Our technology enhances knowledge of the genetic foundations of production, reproduction, and health for a wide variety of applications and species. Illumina\u2019s next-generation sequencing is particularly useful in agricultural research, where genomes can be complex and prior knowledge scarce. NGS is also used to identify pathogens. It allows tracking of microbial or viral adaptation over short periods of time, both in the laboratory and in the environment.<\/p>\n\n\n\n Are you ready for a genotyping game changer? The evolution of genotyping has been extraordinary over the years starting from lab-made PCR reactions and hand-poured gels to commercially purchased master mixes and end-point PCR reading systems. Although the latter enabled greater throughput without extensively reducing hands-on time, LGC (now incorporating Douglas Scientific\u00ae and Biosearch Technologies) is introducing simplified and automated genotyping workflow solutions that can generate up to 23,000 data points per day with minimal hands-on time at a greater cost savings. Whether you focus on trait development, routine screening, zygosity, marker development, or other medium to high-throughput workflows, please join us at our upcoming seminar by registering today to learn more about how our innovative technologies can make your life simpler.<\/p>\n\n\n\n
\n <\/figure>\n<\/div>\n\n\n <\/div>\n\n\n <\/div>\n \n\n\n\nThursday, January 30th from 9:30 AM-3:30 PM (l<\/strong>unch provided if registered<\/a>).<\/strong><\/h3>\n\n\n\n
Stephens Room (Room 3503), Thomas Hall, Main Campus<\/strong><\/h3>\n\n\n\n
2019<\/h2>\n\n\n\n
<\/figure>\n\n\n\n10X Genomics & Illumina Joint Seminar<\/h3>\n\n\n\n
Hosted by the NC State Genomic Sciences Laboratory and the Genetics & Genomics Initiative<\/h4>\n\n\n\n
Wednesday, December 4th from 10 AM \u2013 12 PM in the Stephens Room (3503 Thomas Hall). Refreshments Provided.<\/h3>\n\n\n\n
Please REGISTER<\/strong> for this event here<\/a>.<\/h4>\n\n\n\n
10:00-11:00am<\/strong> \u2013 Nirav Patel, MS, Science & Technology Advisor, 10x Genomics \u2013 \u201cResolving Biology with Single Cell multi-omics and Spatial Transcriptomics\u201d<\/h4>\n\n\n\n
11:00-12:00pm<\/strong>\u2013 Matt Angel, Executive Sequencing & Data Analysis Specialist, Illumina \u2013 \u201cSequencing Considerations of Single Cell Analysis & Emerging Applications\u201d<\/h4>\n\n\n\n
Illumina DNA Workshop Using the Nextera\u2122 DNA Flex Library Preparation Kit on the NovaSeq 6000<\/h3>\n\n\n\n
<\/figure>\n\n\n\nTHIS WORKSHOP IS CURRENTLY FULL \u2013 Please email us for inquiries on waitlisting for future workshops.<\/h3>\n\n\n\n
Hosted by the NCSU BIT Program and the Genomic Sciences Laboratory<\/h4>\n\n\n\n
Wednesday, August 14th (9am-4pm) \u2013 Thursday, August 15th (9am-12:00pm); Jordan Hall Lab Room 6136<\/h4>\n\n\n\n
<\/figure>\n\n\n\nWhole Genome Sequencing Technologies using Oxford Nanopore Technologies to enable high quality reference genomes.<\/h3>\n\n\n\n
Wednesday, May 8th from 1:30-3:00 PM (Stephens Room \u2013 3503 Thomas Hall).<\/h4>\n\n\n\n
Illumina DNA Workshop Using the Nextera\u2122 DNA Flex Library Preparation Kit on the NovaSeq 6000<\/h3>\n\n\n\n
<\/figure>\n\n\n\nTHIS WORKSHOP IS CURRENTLY FULL \u2013 Please email us for inquiries on waitlisting for future workshops.<\/h3>\n\n\n\n
Hosted by the NCSU BIT Program and the Genomic Sciences Laboratory<\/h4>\n\n\n\n
Monday, May 13th (9am-4pm) \u2013 Tuesday, May 14th (9am-12:00pm); Jordan Hall Lab Room 6136<\/h4>\n\n\n\n
How Hi-C is Transforming Genome and Metagenome Assembly<\/h3>\n\n\n\n
<\/figure>\n\n\n\nThursday, March 28th from 12:00-1:00 PM (Stephens Room \u2013 3503 Thomas Hall).<\/h4>\n\n\n\n
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2018<\/h2>\n\n\n\n
CLC Genomics Training Workshop<\/strong><\/h3>\n\n\n\n
<\/figure>\n\n\n\nFriday, October 26th, from 9:00 am \u2013 5:00 pm (l<\/strong>unch provided if registered<\/a>).<\/strong><\/h3>\n\n\n\n
Stephens Room (Room 3503), Thomas Hall, Main Campus<\/strong><\/h3>\n\n\n\n
BioRAD Power PCR Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nTuesday, October 23rd from 11:00AM \u2013 1:00 PM<\/h3>\n\n\n\n
Stephens Room (3503 Thomas Hall)<\/h3>\n\n\n\n
Lunch Provided<\/h3>\n\n\n\n
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Pacific Biosciences Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nThursday, October 18th from 11:30 \u2013 1:30 pm<\/h3>\n\n\n\n
Stephens Room (3503 Thomas)<\/h3>\n\n\n\n
Refreshment & Dessert Provided (bring your lunch)<\/h3>\n\n\n\n
Please Register Here<\/a><\/h3>\n\n\n\n
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<\/figure>\n\n\n\nTWIST Bioscience Lunch & Learn Seminar<\/h3>\n\n\n\n
Thursday, October 11th 12:00 \u2013 1:00 PM<\/h3>\n\n\n\n
Stephens Room (3503 Thomas Hall)<\/h3>\n\n\n\n
Lunch Provided (Limited Seating)<\/h3>\n\n\n\n
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Metabolomics Workshop<\/h3>\n\n\n\n
<\/figure>\n\n\n\nThursday, April 19th from 10:30 AM \u2013 2:30 PM (Stephens Room, 3503 Thomas Hall)<\/h4>\n\n\n\n
Lunch will be provided to registered participants \u2013 click here to register<\/a>.<\/h4>\n\n\n\n
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From Contigs to Chromosomes: how Hi-C is transforming genome and metagenome assembly<\/h3>\n\n\n\n
<\/figure>\n\n\n\nFriday, March 2nd 2:30 \u2013 3:30 PM (Stephens Room \u2013 3503 Thomas Hall) \u2013 refreshments provided.<\/h4>\n\n\n\n
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Dovetail Genomics Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nWednesday, February 14th 10:30 \u2013 11:30 PM (Stephens Room \u2013 3503 Thomas Hall)<\/h4>\n\n\n\n
2017<\/h2>\n\n\n\n
ThermoFisher Scientific CRISPR Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nWednesday, November 29th 12:00 \u2013 1:00 PM (Stephens Room \u2013 3503 Thomas Hall)<\/h4>\n\n\n\n
Lunch Provided<\/strong> for Registered Participants (Register Here<\/a>)<\/h4>\n\n\n\n
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<\/figure>\n\n\n\nNRGene Seminar<\/h3>\n\n\n\n
Wednesday, November 8th from 2:00-4:00 PM in the Stephens Room (3503 Thomas Hall). <\/h4>\n\n\n\n
Refreshments will be provided \u2013 please RSVP to David Neuman (ndavid@nrgene.com)<\/strong><\/h4>\n\n\n\n
Moving beyond a single reference genome: GenoMAGIC, a novel solution to describe and manage genomic variation.<\/h4>\n\n\n\n
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CLC Genomics Training Workshops<\/strong><\/h3>\n\n\n\n
<\/figure>\n\n\n\nWednesday-Thursday, August 30-31, from 8:00 am \u2013 5:00 pm (l<\/strong>unch provided if registered).<\/strong><\/h3>\n\n\n\n
Day One (August 30th) \u2014 Toxicology Auditorium (Room 2014), Toxicology Building, Centennial Campus (see <\/a>Event Flyer<\/a>)<\/strong><\/h3>\n\n\n\n
Day Two (August 31st) \u2014 Stephens Room (Room 3503), Thomas Hall, Main Campus (see Event Flyer<\/a>)<\/strong><\/h3>\n\n\n\n
Please register for this event by email: khushbu.kariwala@qiagen.com<\/a><\/strong><\/h3>\n\n\n
BD Genomics Single Cell Sequencing Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nWednesday, March 22nd from 10:00 am \u2013 11:00 am<\/h4>\n\n\n\n
Room 3503 Thomas Hall (Stephen\u2019s Room)<\/h4>\n\n\n\n
Featured Speaker<\/strong>: John Harrington, Technical Applications Scientist, BD Genomics<\/h4>\n\n\n\n
Please RSVP <\/strong>to: <\/strong>Fran Moses, BD Single Cell Business Partner \u2013 fran.moses@bd.com<\/a><\/h3>\n\n\n\n
Utilizing FACS to Resolve Gene Expression Data with Precision<\/strong><\/h4>\n\n\n\n
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2016<\/h2>\n\n\n\n
ThermoFisher\/Applied Biosystems Technical Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nTuesday, November 15th, 2016 from 11:00 am \u2013 12:00 pm<\/h4>\n\n\n\n
Thomas Hall, Room 3503 (Stephens Room) \u2013 Lunch provided! <\/strong>Please register Here<\/a> for the event.<\/h4>\n\n\n\n
Featured Speaker:<\/strong> Lawrence Yoon<\/h4>\n\n\n\n
Explore the world of RNA<\/strong><\/h4>\n\n\n\n
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Illumina Agricultural Genomics Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nThursday October 27th, 11:00 am \u2013 12:00 pm<\/h4>\n\n\n\n
Thomas Hall, Room 3503 (Stephens Room) \u2013 Lunch provided!<\/h4>\n\n\n\n
Featured Speaker:<\/strong> Ryan Rapp, Associate Director, Agrigenomics, Illumina, Inc.<\/h4>\n\n\n\n
Limited Space available! Please register at event registration. illumina.com\/NCSTATE<\/a><\/h4>\n\n\n\n
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LGC Genomics Seminar<\/h3>\n\n\n\n
<\/figure>\n\n\n\nThursday, October 13th 11:00 am \u2013 12:30 pm<\/h4>\n\n\n\n
Thomas Hall, Room 3503 (Stephens Room) \u2013 Lunch provided!<\/h4>\n\n\n\n
Please register here<\/a> for the event!<\/h4>\n\n\n\n
Featured Speaker:<\/strong> Dr. Niko Tsatsos, LGC Genomics Field Application Scientist<\/h4>\n\n\n\n
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