Dye terminator sequencing is the modern variant of the dideoxy chain termination sequencing method first pioneered by Dr. Frederick Sanger in 1977 (Nobel Prize in Chemistry). The modern method utilizes fluorescent-labeled dideoxy nucleotide chain terminators for use in a single sequencing reaction. During strand synthesis, when polymerase encounters a labeled nucleotide, the strand synthesis is terminated. Use of normal dNTPs in molar excess allows for generation of billions of strands (for each base addition), each labeled with a 3\u2019 fluorescent dideoxy terminator. DNA sequencers separate these strands using capillary electrophoresis, and a laser scanner detects and records the dye fluorescence, outputting the data as a trace chromatogram. This technology produces high-quality reads up to 850 bp, but is unsuitable for high-throughput projects.<\/p>\n\n\n\n