{"id":757,"date":"2022-10-07T12:29:29","date_gmt":"2022-10-07T16:29:29","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=757"},"modified":"2025-06-13T13:40:38","modified_gmt":"2025-06-13T17:40:38","slug":"ngs-library-prep","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","title":{"rendered":"NGS Library Prep"},"content":{"rendered":"\n\n\n\n<div class=\"ncst-fancy-paragraph-fifty is-text wp-block-ncst-fancy-paragraph\">\n                  <p class=\"fancy-paragraph__text fancy-paragraph-left\">\n            The GSL offers a variety of sample library preparation services for Next Generation Sequencing on both Illumina and PacBio platforms:<br><br><strong>Illumina Whole Genome Shotgun DNA<\/strong> <strong>libraries \u2013<\/strong> <em>Illumina Truseq Nano kit, up to 96 indexes<\/em>\n          <\/p>\n                \n<div class=\"wp-block-ncst-fp-accompaniment\">\n    \n<div class=\"wp-block-ncst-fp-image\">\n  <figure class=\"fancy-paragraph__image-container fancy-paragraph__image-landscape\">\n          <img loading=\"lazy\" width=\"658\" height=\"438\" \n        decoding=\"async\"\n        class=\"fp-image wp-image-759\"\n        src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM.png\"\n         alt=\"\" srcset=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM.png 658w, https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM-300x200.png 300w\" sizes=\"auto, (max-width: 658px) 100vw, 658px\" \/>\n          <\/figure>\n<\/div>\n\n\n  <\/div>\n\n\n              <\/div>\n      \n\n\n\n<p><strong>Illumina RNA libraries <\/strong>\u2013 <em>NEB NEBNext Ultra II Direction RNA kit, polyA enriched, up to two, 96 index sets; 192 total. Contact us for alternate ribodepletion options and pricing.<\/em><\/p>\n\n\n\n<p><strong>Illumina 16S\/ITS Amplicon libraries <\/strong>\u2013 <em>16S v3\/v4 region, ITS1 region; pricing by block of 1-48 or&nbsp;49-96 samples, with up to 384 Nextera indexes. Due to our submission volume, we currently do not offer&nbsp;custom amplicon library prep as a service, however if users provide us with amplicons that have a complimentary overhang for Illumina Nextera adapters we may be able to process using our existing workflow. Contact us for more information.<\/em><\/p>\n\n\n\n<p><strong>Illumina Hi-C library prep<\/strong><em>\u2013 Library prep from fresh\/frozen tissue samples, utilizing a Phase Genomics&nbsp;<\/em> <em>Hi-C prep kit. Tissue submissions must BSL1 compliant.<\/em><\/p>\n\n\n\n<p><strong>10X Genomics 3\u2019 Single Cell library prep <\/strong><em>\u2013Local\/regional submissions of live cells only &#8211; will not<\/em>  <em>accept delivery shipment. Samples must comply with our BSL1 level requirements. Due to the nature of<\/em> <em>SC prep, in person coordination with GSL staff is required for cell viability counts. Contact Us for more<\/em> <em>information.<\/em><\/p>\n\n\n\n<p><strong>PacBio DNA libraries<\/strong> \u2013 <em>PacBio SMRTbell DNA library prep, 4-10&nbsp;<\/em><em>kb or 15-20 kb fragment options<\/em><\/p>\n\n\n\n<p><strong>PacBio Iso-Seq RNA libraries <\/strong>\u2013 <em>Isoseq Transcriptome library, 1 <\/em><em>size fraction<\/em><br><em>&nbsp; &nbsp; &nbsp; &nbsp;<\/em><br><strong>PacBio Microbial Indexed DNA libraries <\/strong>\u2013 <em>up to 12 indexes<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Illumina RNA libraries \u2013 NEB NEBNext Ultra II Direction RNA kit, polyA enriched, up to two, 96 index sets; 192 total. Contact us for alternate ribodepletion options and pricing. Illumina&hellip;<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":750,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"useCTA\":false,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageID\":54,\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-757","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NGS Library Prep - Genomic Sciences Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NGS Library Prep - Genomic Sciences Laboratory\" \/>\n<meta property=\"og:description\" content=\"Illumina RNA libraries \u2013 NEB NEBNext Ultra II Direction RNA kit, polyA enriched, up to two, 96 index sets; 192 total. Contact us for alternate ribodepletion options and pricing. Illumina&hellip;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/\" \/>\n<meta property=\"og:site_name\" content=\"Genomic Sciences Laboratory\" \/>\n<meta property=\"article:modified_time\" content=\"2025-06-13T17:40:38+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/ngs-library-prep\\\/\",\"url\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/ngs-library-prep\\\/\",\"name\":\"NGS Library Prep - Genomic Sciences Laboratory\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/#website\"},\"datePublished\":\"2022-10-07T16:29:29+00:00\",\"dateModified\":\"2025-06-13T17:40:38+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/ngs-library-prep\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/ngs-library-prep\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/ngs-library-prep\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Flow Cytometry\",\"item\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Genomics\",\"item\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/other-resources\\\/genomics\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"NGS Library Prep\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/#website\",\"url\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/\",\"name\":\"Genomic Sciences Laboratory\",\"description\":\"Just another Research and Innovation site\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/research.ncsu.edu\\\/gsl\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"NGS Library Prep - Genomic Sciences Laboratory","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","og_locale":"en_US","og_type":"article","og_title":"NGS Library Prep - Genomic Sciences Laboratory","og_description":"Illumina RNA libraries \u2013 NEB NEBNext Ultra II Direction RNA kit, polyA enriched, up to two, 96 index sets; 192 total. Contact us for alternate ribodepletion options and pricing. Illumina&hellip;","og_url":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","og_site_name":"Genomic Sciences Laboratory","article_modified_time":"2025-06-13T17:40:38+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","url":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","name":"NGS Library Prep - Genomic Sciences Laboratory","isPartOf":{"@id":"https:\/\/research.ncsu.edu\/gsl\/#website"},"datePublished":"2022-10-07T16:29:29+00:00","dateModified":"2025-06-13T17:40:38+00:00","breadcrumb":{"@id":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/research.ncsu.edu\/gsl\/"},{"@type":"ListItem","position":2,"name":"Flow Cytometry","item":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/"},{"@type":"ListItem","position":3,"name":"Genomics","item":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/"},{"@type":"ListItem","position":4,"name":"NGS Library Prep"}]},{"@type":"WebSite","@id":"https:\/\/research.ncsu.edu\/gsl\/#website","url":"https:\/\/research.ncsu.edu\/gsl\/","name":"Genomic Sciences Laboratory","description":"Just another Research and Innovation site","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/research.ncsu.edu\/gsl\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/pages\/757","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/users\/1127"}],"replies":[{"embeddable":true,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/comments?post=757"}],"version-history":[{"count":8,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/pages\/757\/revisions"}],"predecessor-version":[{"id":1448,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/pages\/757\/revisions\/1448"}],"up":[{"embeddable":true,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/pages\/750"}],"wp:attachment":[{"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/media?parent=757"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.ncsu.edu\/gsl\/wp-json\/wp\/v2\/tags?post=757"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}