{"id":757,"date":"2022-10-07T12:29:29","date_gmt":"2022-10-07T16:29:29","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=757"},"modified":"2024-05-06T10:19:50","modified_gmt":"2024-05-06T14:19:50","slug":"ngs-library-prep","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/other-resources\/genomics\/ngs-library-prep\/","title":{"rendered":"NGS Library Prep"},"content":{"rendered":"\n\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"658\" height=\"438\" src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM.png\" alt=\"\" class=\"wp-image-759\" srcset=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM.png 658w, https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/Screen-Shot-2022-10-07-at-12.29.49-PM-300x200.png 300w\" sizes=\"auto, (max-width: 658px) 100vw, 658px\" \/><\/figure><\/div>\n\n\n<p>The GSL offers a variety of sample library preparation services for Next Generation Sequencing on both Illumina and PacBio platforms:<\/p>\n\n\n\n<p><strong>Illumina Whole Genome Shotgun DNA<\/strong> <strong>libraries \u2013<\/strong> <em>Illumina Truseq Nano kit<\/em><em>, up to 96 indexes<\/em><\/p>\n\n\n\n<p><strong>Illumina RNA libraries <\/strong>\u2013 <em>NEB NEBNext Ultra II Direction RNA kit, polyA enriched, up to two, 96 index sets; 192 total. Contact us for alternate ribodepletion options and pricing.<\/em><\/p>\n\n\n\n<p><strong>Illumina 16S\/ITS Amplicon libraries <\/strong>\u2013 <em>16S v3\/v4 region, ITS1 region; pricing by block of 1-48 or\u00a049-96 samples, with up to 384 Nextera indexes. Due to our submission volume, we currently do not offer\u00a0custom amplicon library prep as a service, however if users provide us with amplicons that have a complimentary overhang for Illumina Nextera adapters we may be able to process using our existing workflow. Contact us for more information.<\/em><\/p>\n\n\n\n<p><strong>Illumina Hi-C library prep<\/strong><em>\u2013 Library prep from fresh\/frozen tissue samples, utilizing a Phase Genomics&nbsp;<\/em> <em>Hi-C prep kit. Tissue submissions must BSL1 compliant.<\/em><\/p>\n\n\n\n<p><strong>10X Genomics 3\u2019 Single Cell library prep <\/strong><em>\u2013Local\/regional submissions of live cells only &#8211; will not<\/em>  <em>accept delivery shipment. Samples must comply with our BSL1 level requirements. Due to the nature of<\/em> <em>SC prep, in person coordination with GSL staff is required for cell viability counts. Contact Us for more<\/em> <em>information.<\/em><\/p>\n\n\n\n<p><strong>PacBio DNA libraries<\/strong> \u2013 <em>PacBio SMRTbell DNA library prep, 4-10&nbsp;<\/em><em>kb or 15-20 kb fragment options<\/em><\/p>\n\n\n\n<p><strong>PacBio Iso-Seq RNA libraries <\/strong>\u2013 <em>Isoseq Transcriptome library, 1 <\/em><em>size fraction<\/em><br><em>&nbsp; &nbsp; &nbsp; &nbsp;<\/em><br><strong>PacBio Microbial Indexed DNA libraries <\/strong>\u2013 <em>up to 12 indexes<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The GSL offers a variety of sample library preparation services for Next Generation Sequencing on both Illumina and PacBio platforms: Illumina Whole Genome Shotgun DNA libraries \u2013 Illumina Truseq Nano&hellip;<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":750,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"useCTA\":false,\"imageID\":54,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-757","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.3 - 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