{"id":866,"date":"2022-10-20T09:03:25","date_gmt":"2022-10-20T13:03:25","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=866"},"modified":"2022-12-01T09:20:49","modified_gmt":"2022-12-01T14:20:49","slug":"assay-design","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/allele-specific-kasp-genotyping\/assay-design\/","title":{"rendered":"KASP Assay Design"},"content":{"rendered":"\n<p>The user is responsible for their individual assay design and purchasing assays and mastermix. There are two mastermix + assay options available for genotyping SNPs. See <a href=\"https:\/\/www.biosearchtech.com\/products\/pcr-reagents-kits-and-instruments\/pcr-probes-and-assays\/kasp-genotyping-panels?_gl=1*1iom60g*_gcl_aw*R0NMLjE2NTk5NzY3MDkuQ2p3S0NBanc2TUtYQmhBNUVpd0FOV0xPREZjTlVybkZUOFYyV0xlT3RVdXFad3ZoRmI3OURpeFV2MzJUVFlqV0VVUWk0dTdPTWk4SlNCb0N0aklRQXZEX0J3RQ..\">here<\/a> for predesigned KASP assays available from LGC.&nbsp;<\/p>\n\n\n\n<p>LGC KASP By Design Service:<\/p>\n\n\n\n<p class=\"is-style-default\">KASP by Design (KBD) service provides three KASP primers that are specific to the SNP or InDel of interest. The user is responsible for contacting LGC (<a href=\"mailto:Genomics.americas@lgcgroup.com\">Genomics.americas@lgcgroup.com<\/a>) for pricing and assay design.&nbsp; LGC will ship the user three target-specific primers for an assay combined into a single tube. The aliquot contains enough for 5,000 reactions at 5 \u00b5L total reaction volume. The user is also responsible for purchasing KASP master mix from LGC.<\/p>\n\n\n\n<p>3CR Biosciences PACE Assay Design:<\/p>\n\n\n\n<p>3CR Biosciences offers a free assay design service that returns the designed oligo sequences and a prepopulated ordering form that can be submitted to IDT for purchasing primers. Please email <a href=\"mailto:support@3crbio.com\">support@3crbio.com<\/a> for more information. The user is also responsible for purchasing PACE master mix from 3CR.&nbsp;<\/p>\n\n\n\n<p><strong>Reaction Assembly For Meridian<\/strong><\/p>\n\n\n\n<p>For dispensing in 384 well plate:<br>2X Master Mix= 814 uL<br>72X Assay Mix= 23 uL<br>Water= 791 uL<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The user is responsible for their individual assay design and purchasing assays and mastermix. There are two mastermix + assay options available for genotyping SNPs. See here for predesigned KASP&hellip;<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":850,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"pageIntro\":\"\",\"imageID\":54,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-866","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>KASP Assay Design - Genomic Sciences Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.ncsu.edu\/gsl\/allele-specific-kasp-genotyping\/assay-design\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"KASP Assay Design - Genomic Sciences Laboratory\" \/>\n<meta property=\"og:description\" content=\"The user is responsible for their individual assay design and purchasing assays and mastermix. 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