{"id":900,"date":"2022-10-20T09:39:44","date_gmt":"2022-10-20T13:39:44","guid":{"rendered":"https:\/\/research.ncsu.edu\/gsl\/?page_id=900"},"modified":"2022-12-01T09:23:13","modified_gmt":"2022-12-01T14:23:13","slug":"rna-extractions","status":"publish","type":"page","link":"https:\/\/research.ncsu.edu\/gsl\/dna-and-rna-extraction-guidelines\/rna-extractions\/","title":{"rendered":"RNA Extractions"},"content":{"rendered":"\n<p>The GSL offers low-throughput (<em>by hand<\/em>) extraction of RNA from tissues. Please see our pricing page for more information on service costs.<\/p>\n\n\n\n<p>The GSL is a Biosafety Level 1 genomics core managed under the regulations of NC State University teaching labs. We <strong>CANNOT<\/strong> <strong>accept human blood OR <\/strong><strong>pathogenic<\/strong><strong> tissue samples<\/strong>, due to potential risk to our staff and students. The GSL will not be held responsible for samples shipped to us in violation of this policy. For other providers, please contact us directly.<\/p>\n\n\n\n<p><strong>Submission Requirements<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"733\" height=\"253\" src=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/RNA-extractions.jpg\" alt=\"\" class=\"wp-image-901\" srcset=\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/RNA-extractions.jpg 733w, https:\/\/research.ncsu.edu\/gsl\/files\/2022\/10\/RNA-extractions-300x104.jpg 300w\" sizes=\"auto, (max-width: 733px) 100vw, 733px\" \/><\/figure>\n\n\n\n<p>The GSL uses Qiagen Rneasy mini kits to perform on column total RNA extractions. Please specify species and tissue type in the submission form so we can use the best kit for these isolations (Plant, Fibrous Tissue, Lipid Tissue, etc.).&nbsp;<\/p>\n\n\n\n<p>Please submit tissue samples in <em>RNAlater<\/em> (on wet ice) or snap frozen (on dry ice) in labeled, 1.7 mL tubes. See table for the recommended amounts of cells or tissue to provide. If using <em>RNAlater<\/em>, please reference the protocol here for proper use.<\/p>\n\n\n\n<p>If NGS library preparation and sequencing is to be completed using these samples, a <strong>separate NGS submission form <\/strong>should be entered into our Lab Management System.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The GSL offers low-throughput (by hand) extraction of RNA from tissues. Please see our pricing page for more information on service costs. The GSL is a Biosafety Level 1 genomics&hellip;<\/p>\n","protected":false},"author":1127,"featured_media":0,"parent":885,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"ncst_custom_author":"","ncst_show_custom_author":false,"ncst_dynamicHeaderBlockName":"ncst\/floating-box-header","ncst_dynamicHeaderData":"{\"backgroundColor\":\"red_400\",\"boxPosition\":\"left\",\"ctaNum\":\"one\",\"imageID\":54,\"imageURL\":\"https:\/\/research.ncsu.edu\/gsl\/files\/2022\/07\/about-dna.jpg\",\"imageAlt\":\"blue dna\"}","ncst_content_audit_freq":"","ncst_content_audit_date":"","ncst_content_audit_display":false,"ncst_backToTopFlag":"","footnotes":""},"tags":[],"class_list":["post-900","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>RNA Extractions - Genomic Sciences Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.ncsu.edu\/gsl\/dna-and-rna-extraction-guidelines\/rna-extractions\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RNA Extractions - Genomic Sciences Laboratory\" \/>\n<meta property=\"og:description\" content=\"The GSL offers low-throughput (by hand) extraction of RNA from tissues. 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