3i Marianas Spinning Disk Confocal
Please read the facility policy and contact CMIF to set up an appointment.
The 3i Marianas is a CSU-X1 A1 Spinning Disk Confocal attached to a Zeiss Axio Observer 7 microscope with a range of long working distance lenses including a 63x water immersion objective. The system is optimized for speed and features a Hamamatsu ImagEM X2 back-thinned EM CCD camera with 512 x 512 pixels and capture speed up to 70 fps. It also has an Ablate!™ 532 module with 532nm pulsed laser for photo-activation and ablation and a VectorM™ VIS High Speed Point Scanner for photomanipulation techniques including FRAP, photoactivation and photoablation.
Image and Video Gallery
See confocal workstation gallery page
Laser Lines for Excitation
- 405 nm solid state laser
- 488 nm solid state laser
- 561 nm solid state laser
Dichroics and Emission Filters
- CSU-X1 dichroic for 488/561 excitation with dual emitter
- CSU-X1 dichroic for 405/488/561/640 excitation with quad emitter
- bandpass filter 617/73
- bandpass filter 525/45
Laser Ablation
Ablate!™ 532 module with 532nm pulsed laser (pulse energy > 60 microJ at 200 Hz) for photo-activation and ablation and VectorM™ VIS High Speed Point Scanner.
Camera
Hamamatsu ImagEM X2 back-thinned EM CCD camera with 512 x 512 pixels, pixel size 16μm, frame rate 70 fps.
Microscope and Accessories
Zeiss Axio Observer 7 inverted microscope with motorized, programmable x,y,z stage, additional piezo z-stage, joystick and touch panel docking control station. A definite focus (Version 2) attachment corrects for focus drifts during overnight time lapse experiments.
Objectives
| Magnification | N.A. Immersion | Type | Working Distance |
|---|---|---|---|
| 20x | 0.5 dry | EC Plan Neofluar | 2mm |
| 40x | 1.2 W | C-Apochromat | 0.28 mm |
| 63x | 1.15 W | LD C-Apochromat | 0.60 mm |
Software
SlideBook™ 6 for Windows 7. Ratio Imaging Module for Slidebook™ Automated ratio imaging detection for capture, automated ratio calculation, ratio calibration guide, pixel by pixel or arbitrary area ratio calculations, real-time ratio calculations, real-time ratio graphing and more. FRET Module for SlideBook™ Includes tools to measure FRET on both a pixel-by-pixel and region basis; uses filter-based FRET computation and bleed-through correction methods including ratiometric FRET, FRETN, E-FRET, and acceptor photobleaching. Offline version with all features is available in CMIF.