Andor Dragonfly 302 Spinning Disk Confocal
Please read the facility policy and contact CMIF to set up an appointment.
The Andor Dragonfly 302 Spinning disk confocal is attached to a Nikon Eclipse Ti microscope and has a range of oil immersion objectives and TIRF (Total Internal Reflection Fluorescence) capability. The system is optimized for resolution and has two cameras: Zyla 4.2 Megapixel sCMOS camera and iXon Ultra 888 Ultra EM CCD Camera (1024 x 1024 pixels). It features Andor Micropoint (point scanner) with tunable pulsed N2 laser for organelle ablation and high precision photoactivation.
Image and Video Gallery
See confocal workstation gallery page
Laser Lines for Excitation
- 488 nm solid state smart diode laser, 50 mW
- 561 nm solid state smart OPSS laser, 100 mW
- 637 nm solid state smart diode laser, 140 mW
Dichroics and Emission Filters
- 405/488/561/640 quad excitation dichroic
- bandpass filter 525/50 nm
- bandpass filter 600/50 nm
- bandpass filter 725/40 nm
Laser Ablation
MicroPoint Galvo Position control; N2 pulsed laser (dye pump) 337 nm, 3 ns, 100 μJ, 20 Hz, with pulse controller, dyes: 551 nm and 590 nm for precise ablation/ photoactivation at 551 nm and 590 nm.
Cameras
- Andor iXon DU888U3-CS0-BV EM CCD camera, 1024 x 1024 pixels, pixel size 13 x 13 μm; Readout Rate 30 MHz; Frame Rate 26 – 9690 fps; QEmax > 90%.
- Zyla PLUS 4.2 Megapixel sCMOS camera; QE 80%.
Mosaic 3
Mosaic 3 random access active illumination device, ~20 mm primary image plane field of illumination, pE-300 white LED illumination unit provides a broad spectrum of
illumination, UV peak at 400nm, covering the excitation bands of common fluorescent stains.
Microscope and Accessories
Nikon Eclipse TI inverted microscope with motorized x,y,z stage, additional piezo z-stage, joystick and touch panel docking control station. Perfect Focus attachment corrects for focus drifts during overnight time lapse experiments. Stage top incubation chamber with temperature, humidity and CO2 control (Okolab).
Objectives
| Magnification | N.A. Immersion | Type | Working Distance |
|---|---|---|---|
| 40x | 1.3 oil | CFI Plan Fluor | 0.2 mm |
| 60x (phase) | 1.4 oil | CFI Plan Apochromat | 0.13 mm |
| 60x (TIRF) | 1.49 oil | CFI Apochromat (TIRF) | 0.12 mm |
| 100x (phase) | 1.45 oil | CFI Plan Apochromat | 0.13 mm |
Software
Fusion and iQ3 for image acquisition. Imaris 9.0 for image analysis and data presentation.