Keyence BZ-X810
Please read the facility policy and contact CMIF to set up an appointment.
The Keyence BZ-X810 is a fluorescence microscope for streamlined high-throughput image acquisition and batch analysis of large data sets. It supports fluorescence as well as brightfield imaging and phase contrast. The camera can be switched from monochrome to 3 CCD color mode with accurate color reproduction for histology applications. Optical sectioning in fluorescence mode is achieved by structured illumination. The system features a motorized stage, a large variety of stage inserts and comes with an incubation chamber for temperature and CO2 regulation that accommodates a variety of vessels including 96 well plates. Please refer to the manufacturer’s webpage for a full list of capabilities and application examples. Our system comes with all hardware and software modules listed in the brochure. Details about our fluorescence filter sets and objective lenses are listed below.
Image and Video Gallery
Filter Sets
- DAPI: Excitation: 360/40 nm; Emission: 460/50 nm; Dichroic 400 nm
- GFP: Excitation: 470/40 nm; Emission: 525/50 nm; Dichroic 495 nm
- TRITC: Excitation: 545/25 nm; Emission: 605/70 nm; Dichroic 565 nm
- Texas Red: Excitation: 560/40 nm; Emission: 630/75 nm; Dichroic 585 nm
- Cy5: Excitation: 620/60 nm; Emission: 700/75 nm; Dichroic 660 nm
Objectives
| Magnification | N.A. Immersion | Type | Working Distance |
|---|---|---|---|
| 2x | 0.1 | Plan APO | 8.5 mm |
| 10x | 0.45 | Plan APO | 4 mm |
| 20x | 0.75 | Plan APO | 1 mm |
| 60x | 1.4 oil | Plan APO | 0.13 |
| 20x (phase) | 0.45 | Plan Fluorite LD PH | 8.2-6.9 mm |
| 40x (phase) | 0.60 | Plan Fluorite LD PH | 3.6-2.8 mm |
Resources
- Keyence webpage
- Resources for NC State students, faculty and staff (login required)