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Office of Research and Innovation
Cellular and Molecular Imaging Facility

Zeiss Lightsheet Z.1 Workstation

Please read the facility policy and contact CMIF to set up an appointment.

This microscope is ideal for multiview and long term live imaging of medium to large transparent specimen. The specimen is embedded in low melting point agarose and suspended in a medium-filled environmental chamber. A sheet of laser light is created using 2 illumination objectives that are positioned perpendicular to the detection objective. This way only a narrow plane of the specimen is illuminated. Optical sectioning is achived by moving the specimen relative to the illumination and detection optics. Images are acquired with 2 s-CMOS PCO.edge cameras.

Image and Video Gallery

See Lightsheet Z.1 Workstation gallery page

Laser lines for excitation

  • 405 nm 50 mW
  • 445 nm 25 mW
  • 488 nm 50 mW
  • 515 nm 20 mW
  • 561 nm 50 mW
  • 638 nm 75 mW

Filter modules

  • DAPI – GFP (beam splitter SBS LP 490; emission filters BP 420-470 and BP 505-545)
  • CFP – YFP (beam splitter SBS LP 510; emission filters BP 460-500 and BP 525-565)
  • GFP – mCherry (beam splitter SBS LP 560; emission filters BP 505-545 and BP 599-660)
  • GFP – DRAQ5 (beam splitter SBS LP 560; emission filters BP 505-545 and LP 660)
  • Universal green-red ((beam splitter SBS LP 560; emission filters SP 550 and LP 585)

Detection objectives

  • 5x  0.16 NA dry
  • 10x 0.5 NA (water dipping)
  • 20x 1 NA (water dipping
  • 40x 1 NA (water dipping)

Suitable specimen

  • zebrafish embryo
  • drosophila embryo, puppa
  • Arabidopsis seedling root
  • anything transparent up to 5mm

Further infomation

Reservation and Rules

Once approved to use the Zeiss Lightsheet Z.1 independently you can reserve time online by visiting the NC State Lab Management portal. Log in with your unity credentials. Navigate to CMIF and select Zeiss Lightsheet Z.1.
There are currently no sign up restrictions for this resource. Please be considerate and communicate with other users of this resource if you need excessive time and/or have to cancel at short notice. Please always sign up. If you forget to do so someone else assumes the instrument is available. In case of conflict the person signed up has priority.