Allen Distinguished Microscopy Seminar

Connecting protein conformational dynamics with function using single molecule fluorescence microscopy

Keith Weninger, Ph.D, Assistant Professor
University Faculty Scholar
Department of Physics, College of Sciences, NCSU

Sponsored By:

ORIED, CALS, COS, CVM & COE.

Abstract: Observing single molecules allows discovery of properties that are typically hidden by averaging over the large number of unsynchronized molecules typical of biological samples. Single molecule fluorescence resonance energy transfer is a method that allows real-time observation of protein and DNA conformational changes with nanometer resolution. I will describe our applications of single molecule fluorescence microscopy to studies of proteins involved in several phenomena including DNA mismatch repair, neurotransmitter release and cancer related processes.