Area(s) of Expertise

Leonard became a Research Specialist at UNC-Chapel Hill in 2006 and worked in the Biomarker Mass Spectrometry facility until arriving at NC State in 2020. Leonard has experience developing mass spectrometry methods for DNA and RNA oligos, DNA damage products, single nucleotides, intact proteins and peptides, natural molecules and metabolites, drugs and metabolites, and environmental contaminants such as PAHs and PFAS.

Publications

• Adrenodoxin alters human cytochrome P450 27A1 structure and reaction efficiency beyond supplying electrons , Archives of Biochemistry and Biophysics (2025)
• Illuminating the mechanistic impacts of an Fe-quaterpyridine functionalized crystalline poly(triazine imide) semiconductor for photocatalytic CO ₂ reduction , Inorganic Chemistry Frontiers (2025)
• Injection of vocal fold lamina propria-derived hydrogels modulates fibrosis in injured vocal folds , Biomaterials Advances (2025)
• Use of Synthetic Standard Peptides in Standardized Digests to Evaluate Both Sample and Instrument Suitability in Proteomics , Journal of Proteome Research (2025)
• Myeloid cell MHC I expression drives CD8+ T cell activation in nonalcoholic steatohepatitis , Frontiers in Immunology (2024)
• The biochemical effects of carotenoids in orange carrots on the colonic proteome in a mouse model of diet-induced obesity , Frontiers in Nutrition (2024)
• Discovery Proteomics and Absolute Protein Quantification Can Be Performed Simultaneously on an Orbitrap-Based Mass Spectrometer , ACS Omega (2023)
• Discovery of a Hybrid System for Photocatalytic CO₂ Reduction via Attachment of a Molecular Cobalt-Quaterpyridine Complex to a Crystalline Carbon Nitride , ACS Applied Energy Materials (2023)
• Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach , Microbial Cell Factories (2023)
• Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study , Separation and Purification Technology (2023)

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