General

Where are you located?

The GSL is located on the second floor (room 2518) of Thomas Hall on the North Campus of NC State University. For map and contact information, visit Contact Us.

What time can I stop by to drop off samples?

Our hours for sample drop-off are 9 AM until 4 PM Monday through Friday. We ask that if you want to stop by during lunch (12-1 PM) that you call ahead to confirm your plans (919-513-3882).

Can I schedule a meeting to discuss a project I had in mind?

Of course! We encourage you to discuss your sequencing project needs with us. Please email or call Andy (919-513-0738; dabaltze@ncsu.edu), Julie (919-513-3882; jabenson@ncsu.edu), Kelly (919-515-3882; kafridey@ncsu.edu), Hannah (919-513-3882; hdhuntle@ncsu.edu), or Caitlin (919-513-3882; ckwotani@ncsu.edu ) to set-up a time.

Do you accept projects from outside of NC State University or outside of the United States?

Yes. We have many customers from all over the country and around the world. Please call or email Andy (919-513-0738; dabaltze@ncsu.edu) for questions about billing concerns.

Are your prices different for NC State customers, other universities, or for businesses?

All U.S. federally-funded parties are charged the same use rates. Other parties or businesses, please contact us to discuss pricing.

Single Tube and Full-Plate Sanger Sequencing

What concentration does my PCR product need to be for Sanger (Capillary) Sequencing?

Please see our Sanger Sequencing Guidelines for additional details.

Do you add primers for Single-Tube Sequencing?

We can add M13F, M13R, T7, or Sp6 primers.

What size tubes do you prefer that I use for my samples?

Please bring your sample in 1.7mL tubes that are clearly labeled.

What time must I drop-off my sequencing samples if I wish same-day service?

Please note that only Gold and Bronze Sequencing are available for next day service. To get next-day delivery, your samples must be dropped-off at the GSL no later than 11:30 PM. In addition to dropping off your samples, you will need to submit your request through our online submission system.

When will I get my results?

If you drop your samples (Gold or Bronze) off before 11:30 AM, you should have results around 5 PM. If you drop off your samples after 11:30, you will have them by 5 PM the next day.

Next Generation Sequencing

How much DNA/RNA do I need for Next Generation Sequencing Library preps?

Although it depends on the type of preps you are interested in, a good starting point is 2 μg of DNA or RNA in a volume of 50 μl (40 ng/μl). If you are unable to prepare 2 μg of DNA or RNA from your sample, please contact the GSL.

What type of Next Generation Sequencing Services do you offer?

We currently house two Illumina MiSeq sequencers, one NextSeq 500, and one PacBio Sequel in our facility. We also provide Illumina HiSeq 2500 sequencing through arrangement with NCSU faculty.

How do you recommend shipping RNA or DNA to your lab?

We recommend shipping DNA overnight with freezer packs. For RNA we recommend shipping on dry ice, with the total RNA either dissolved in nuclease-free water (50 μl), or as a precipitated pellet in 70% ethanol. If the sample is mRNA, please contact the GSL to determine the appropriate volume.

Do you extract RNA for customers?

Yes, depending on several factors. Please contact the GSL for more information.

Bioanalyzer and PippinPrep

What concentration should my sample be for the Bioanalyzer?

For the DNA High Sensitivity Chip and for the RNA Pico chip, please bring your samples at a concentration of 1-5 ng/μl. For the RNA nano chip, samples may be at 50-200 ng/μl.

How much sample do I need to bring for the Bioanalyzer?

Please bring 3-5 μl of each sample at the appropriate concentration in a 1.7 mL tube, labeled clearly.

How do I prepare my sample for the PippinPrep?

Pippen prep samples should be submitted in exactly 30 μl of EB, TE, or water.

Other Questions

Do you let customers use the lab equipment?

Most of our equipment is used only by GSL staff, however, there are certain pieces of equipment that our customers can access after training. Please refer to our list of Other Resources page for more information.

Do you set up qRT-PCR plates?

Our customers set up their own qRT-PCR plates and run the plates themselves. Training on the BioRad CFX384 Real-Time PCR machine is required before you can access the calendar and sign-up to use the machine. Training and instrument use are available through our online submission system.

Do you train customers on how to prepare libraries?

Currently we cannot offer extensive training, due to our increasing submission volume. We can provide a detailed review of relevant protocols, and further Q&A. Please contact the GSL for more information.