Skip to main content
blue dna

Pricing

Note: All prices below are for NC State University faculty and research investigators. For external customers, please reach out (Contact Page) for appropriate pricing and quotes. A standard 3% charge will be added for credit card transactions.

The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. Please contact the GSL Director BEFORE submitting ANY samples or research projects related to the SARS-COV2 pandemic or other samples of pathogenic risk to humans. We will try and point you to a suitable provider if we are unable to provide services directly.

Current GSL Services & Pricing (effective April 3rd, 2023)

Notes: 10X library prep is currently only available for internal users, utilizing cells with BSL1 (nonpathogenic) status. Due to unique cycle settings, 10X libraries require a dedicated flow cell for sequencing. Currently only raw .bcl file delivery available due to specific demultiplexing requirements.

Nucleic Acid Isolation & Quantification

Note: GSL DNA extraction services may not be suitable for NGS applications requiring extremely high molecular weight DNA inputs, such as PacBio or Oxford Nanopore long-read sequencing approaches.

ServiceUnitPrice
RNA Extractionsper 2 samples$98.00
HT Plant DNA extraction (Oktopure)per 96w plate$300.00
HT Plant DNA extraction (Oktopure)per sample$3.50
DNA extractions (Kingfisher) per 96 samples$485.00
LT DNA extractions (Kingfisher)
from 1-24 samples$190.00
FS Picogreen DNA Quant (384)from 97-384 samples$160.00
FS Picogreen DNA Quant (96)from 49-96 samples$125.00
FS Picogreen DNA Quant (48)from 1- 48 samples$80.00
Sample Normalization (96)per plate$34.00
TapeStation DNA Analysis (D1000/D5000 Tapes)per 12 samples 1$64.00
TapeStation High Sensitivity DNA Analysisper 12 samples 1$92.00
TapeStation High Molecular Weight DNA Analysisper 12 samples 1$92.00
BioAnalyzer RNA Analysis – Pico sensitivityper 11 samples 1$68.00
BioAnalyzer RNA Analysis – Nano sensitivityper 12 samples 1$68.00

1 Split pricing is available for half (1-6) and full (7-12) sets of samples. Bioanalyzer RNA chips run 11-12 samples, depending on the sensitivity level (pico vs nano).

Sanger Sequencing & Genotyping

96-well plate

ServiceUnitPrice
Genotyping & Fragment Analysis96 well plate$43.00
Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run96 well plate$390.00
Gold Sequencing – Seq Rxn, Clean Up & Capillary Run96 well plate$300.00
Bronze Sequencing – Clean Up & Capillary Run96 well plate$130.00

Single tube

ServiceUnitPrice
Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Runper sample$6.25
Gold Sequencing – Seq Rxn, Clean Up & Capillary Runper sample$4.75
Bronze Sequencing – Clean Up & Capillary Runper sample$3.00

Next-Generation Sequencing

Illumina Services

ServiceUnitPricing
Illumina DNA and RNA Libraries 1-7per sample 1$150.00
Illumina DNA and RNA Libraries 8-15per sample 1$140.00
Illumina DNA and RNA Libraries 16-89per sample 1$130.00
Illumina DNA and RNA Libraries 90+per sample 1$120.00
Illumina HT DNA Library Prep (96w)per 96w plate$5,000.00
Illumina small RNA Librariesper sample 1$250.00
Hi-C Library prep (PhaseGenomics)per sample 1$1,000.00
16S Amplicon Library Prepper block of 48 or 96 samples$925.00 (1-48) or $1,850.00 (49-96)
10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only)first sample$2,500.00
10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only)per sample (samples 2-8)$1,950.00
MiSeq v2 250 PE NANO flow cellper cell (1M reads 2)$715.00
MiSeq v2 150 bp PE flow cellper cell (12M reads 2)$1,525.00
MiSeq v2 250 bp PE flow cellper cell (12M reads 2)$1,700.00
MiSeq v3 150 bp SR/ 75 bp PE flow cellper cell (20M reads 2)$1,375.00
MiSeq v3 300 bp PE flow cellper cell (20M reads 2)$2,075.00
NextSeq 2000 100SR/ 50 PE P1 flow cellper cell (100M reads 2)$1,250.00
NextSeq 2000 150 PE P1 flow cellper cell (100M reads 2)$1,600.00
NextSeq 2000 300 PE P1 flow cellper cell (100M reads 2)$2,150.00
NextSeq 2000 100SR/ 50 PE P2 flow cellper cell (400M reads 2)$1,925.00
NextSeq 2000 100 PE P2 flow cellper cell (400M reads 2)$3,000.00
NextSeq 2000 150 PE P2 flow cellper cell (400M reads 2)$3,900.00
NextSeq 2000 300 PE P2 flow cellper cell (400M reads 2)$4,200.00
NextSeq 2000 50SR/ 25 PE P3 flow cellper cell (1.2 Billion reads 2)$2,800.00
NextSeq 2000 100SR/ 50 PE P3 flow cellper cell (1.2 Billion reads 2)$4,000.00
NextSeq 2000 100 PE P3 flow cellper cell (1.2 Billion reads 2)$5,400.00
NextSeq 2000 150 PE P3 flow cellper cell (1.2 Billion reads 2)$7,200.00
Per sample Sequencing (NovaSeq S4 150 PE)per sample (40 Million reads3,4)$125.00
NovaSeq 6000 SP 100 bp SR lane ***per lane (400M reads 2,3)$1,925.00
NovaSeq 6000 SP 150 bp PE lane ***per lane (400M reads 2,3)$2,475.00
NovaSeq 6000 SP 100 bp SR/50 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$3,850.00
NovaSeq 6000 SP 100 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$4,600.00
NovaSeq 6000 S4 150 bp PE lane ***per lane (2.5 Billion reads 2,3)$4,650.00
NovaSeq 6000 SP 150 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$4,950.00
NovaSeq 6000 SP 250 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$5,800.00
NovaSeq 6000 S1 100 bp SR/50 PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$6,000.00
NovaSeq 6000 S1 100 bp PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$7,100.00
NovaSeq 6000 S1 150 bp PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$7,600.00
NovaSeq 6000 S2 100 bp SR/50 PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$9,800.00
NovaSeq 6000 S2 100 bp PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$11,800.00
NovaSeq 6000 S2 150 bp PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$12,500.00
NovaSeq 6000 S4 100 bp PE flow cellper cell (10.0 Billion reads 2) – 4 independent lanes$16,650.00
NovaSeq 6000 S4 150 bp PE flow cellper cell (10.0 Billion reads 2) – 4 independent lanes$18,500.00

1 For GSL library preparation, an additional charge for template quality & integrity will be added. Please see our Bioanalyzer & Tapestation pricing for more information.

2 Yield estimates reflect optimum values defined by Illumina and apply to GSL prepared libraries only. We cannot guarantee customer-supplied libraries will meet this estimate.

Lane or block pricing reflects divided user costs, however sequencing turn-around times are subject to sufficient user volume in order to fill the flow cell.

Block sequencing is performed on NovaSeq S4 150 PE lanes, and is only available for select GSL-prepared library options, we cannot offer block pricing options for user prepared libraries, or those not suitable for sequencing at 150×2 PE, or will impact the performance of other customer libraries .

PacBio Services

ServiceUnitPrice
HiFi Sample Size-Selection/Cleanupper sample$160.00
HiFi DNA Library Prep (unbarcoded)per sample$450.00
HiFi Barcoded DNA Library Prepper sample$475.00
PacBio Iso-Seq Library Prep (1 fraction)per sample$500.00
PacBio Sequel IIe Cell per cell$1,800.00
PacBio Sequel IIe Cell (4 Cell Block)per 4 cells$6,000.00

Phage Sequencing

ServiceUnitPrice
Phage Sequencingper sample$225.00
Phage Assemblyper sample$50.00

Miscellaneous Services & Supplies

ServiceUnitPrice
Pippen Prep (1-3 samples)per set$83.00
Pippen Prep ( 4-5 samples)per set$90.00
BioRad CFX384 qPCR run 2,3per plate$25.00
BioRad digital droplet PCR 3per sample$3.75
Misc serviceper hour$80.00
Robotics/instrumentation use 1per hour 1$38.00
BigDye Sanger mix (600 uL)per tube$1,250.00
Ampure XP Beads (10 mL)per tube$310.00
Covaris G Tubesper tube$33.00
Covaris Tubeseach$8.25
qPCR plate & seal for CFX384 2each$7.00
qPCR seal only 2each$2.00
Illumina Library KitInternal Users only, contact for quote.

1 Usage fee applies to the Covaris Ultrasonicator, Qiagen TissueLyzer, Synergy H1 Hybrid Plate Reader, and GenoGrinder 2010 instruments. Users are charged in increments of 0.25 hours, depending on total use time.

2 When running the CFX384 qPCR machine, the user must use BioRad optical seals and qPCR plates. Please contact us before purchasing your own supplies for qPCR to ensure you are using the correct type.

3 This is shared research equipment and available only to local users. The GSL does not perform assays on this equipment as a direct service.