Pricing
Note: All prices below are for all researchers, including those affiliated with NCSU, UNC, Duke, and other federally-funded or non-profit organizations. A standard 3% charge will be added for credit card transactions.
The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. Please contact the GSL Director BEFORE submitting ANY samples or research projects related to the SARS-COV2 pandemic or other samples of pathogenic risk to humans. We will try and point you to a suitable provider if we are unable to provide services directly.
Current GSL Services & Pricing (effective April 3rd, 2023)
Notes: 10X library prep is currently only available for internal users, utilizing cells with BSL1 (nonpathogenic) status. Due to unique cycle settings, 10X libraries require a dedicated flow cell for sequencing. Currently only raw .bcl file delivery available due to specific demultiplexing requirements.
Nucleic Acid Isolation & Quantification
Note: GSL DNA extraction services may not be suitable for NGS applications requiring extremely high molecular weight DNA inputs, such as PacBio or Oxford Nanopore long-read sequencing approaches.
| Service | Unit | Price |
|---|---|---|
| RNA Extractions | per 2 samples | $98.00 |
| HT Plant DNA extraction (Oktopure) | per 96w plate | $300.00 |
| HT Plant DNA extraction (Oktopure) | per sample | $3.50 |
| DNA extractions (Kingfisher) | per 96 samples | $485.00 |
| LT DNA extractions (Kingfisher) | from 1-24 samples | $190.00 |
| FS Picogreen DNA Quant (384) | from 97-384 samples | $160.00 |
| FS Picogreen DNA Quant (96) | from 49-96 samples | $125.00 |
| FS Picogreen DNA Quant (48) | from 1- 48 samples | $80.00 |
| Sample Normalization (96) | per plate | $34.00 |
| TapeStation DNA Analysis (D1000/D5000 Tapes) | per 12 samples 1 | $64.00 |
| TapeStation High Sensitivity DNA Analysis | per 12 samples 1 | $92.00 |
| TapeStation High Molecular Weight DNA Analysis | per 12 samples 1 | $92.00 |
| BioAnalyzer RNA Analysis – Pico sensitivity | per 11 samples 1 | $68.00 |
| BioAnalyzer RNA Analysis – Nano sensitivity | per 12 samples 1 | $68.00 |
1 Split pricing is available for half (1-6) and full (7-12) sets of samples. Bioanalyzer RNA chips run 11-12 samples, depending on the sensitivity level (pico vs nano).
Sanger Sequencing & Genotyping
96-well plate
| Service | Unit | Price |
|---|---|---|
| Genotyping & Fragment Analysis | 96 well plate | $43.00 |
| Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run | 96 well plate | $390.00 |
| Gold Sequencing – Seq Rxn, Clean Up & Capillary Run | 96 well plate | $300.00 |
| Bronze Sequencing – Clean Up & Capillary Run | 96 well plate | $130.00 |
Single tube
| Service | Unit | Price |
|---|---|---|
| Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run | per sample | $6.25 |
| Gold Sequencing – Seq Rxn, Clean Up & Capillary Run | per sample | $4.75 |
| Bronze Sequencing – Clean Up & Capillary Run | per sample | $3.00 |
Next-Generation Sequencing
Illumina Services
| Service | Unit | Pricing |
|---|---|---|
| Illumina DNA and RNA Libraries 1-7 | per sample 1 | $150.00 |
| Illumina DNA and RNA Libraries 8-15 | per sample 1 | $140.00 |
| Illumina DNA and RNA Libraries 16-89 | per sample 1 | $130.00 |
| Illumina DNA and RNA Libraries 90+ | per sample 1 | $120.00 |
| Illumina small RNA Libraries | per sample 1 | $250.00 |
| Hi-C Library prep (PhaseGenomics) | per sample 1 | $1,000.00 |
| 16S Amplicon Library Prep | per block of 48 or 96 samples | $925.00 (1-48) or $1,850.00 (49-96) |
| 10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only) | first sample | $2,500.00 |
| 10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only) | per sample (samples 2-8) | $1,950.00 |
| MiSeq v2 250 PE NANO flow cell | per cell (1M reads 2) | $715.00 |
| MiSeq v2 150 bp PE flow cell | per cell (12M reads 2) | $1,525.00 |
| MiSeq v2 250 bp PE flow cell | per cell (12M reads 2) | $1,700.00 |
| MiSeq v3 150 bp SR/ 75 bp PE flow cell | per cell (20M reads 2) | $1,375.00 |
| MiSeq v3 300 bp PE flow cell | per cell (20M reads 2) | $2,075.00 |
| NextSeq 500 v2 75 bp SR flow cell | per cell (400M reads 2) | $2,500.00 |
| NextSeq 500 v2 150 bp SR/ 75 bp PE flow cell | per cell (400M reads 2) | $4,125.00 |
| NextSeq 500 v2 150 bp PE flow cell | per cell (400M reads 2) | $6,200.00 |
| Per sample Sequencing (NovaSeq S4 150 PE) | per sample (40 Million reads3,4) | $125.00 |
| NovaSeq 6000 SP 100 bp SR lane *** | per lane (400M reads 2,3) | $1,925.00 |
| NovaSeq 6000 SP 150 bp PE lane *** | per lane (400M reads 2,3) | $2,475.00 |
| NovaSeq 6000 SP 100 bp SR/50 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $3,850.00 |
| NovaSeq 6000 SP 100 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $4,600.00 |
| NovaSeq 6000 S4 150 bp PE lane *** | per lane (2.5 Billion reads 2,3) | $4,650.00 |
| NovaSeq 6000 SP 150 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $4,950.00 |
| NovaSeq 6000 SP 250 bp PE flow cell | per cell (800M reads 2) – 2 independent lanes | $5,800.00 |
| NovaSeq 6000 S1 100 bp SR/50 PE flow cell | per cell (1.6 Billion reads 2) – 2 independent lanes | $6,000.00 |
| NovaSeq 6000 S1 100 bp PE flow cell | per cell (1.6 Billion reads 2) – 2 independent lanes | $7,100.00 |
| NovaSeq 6000 S1 150 bp PE flow cell | per cell (1.6 Billion reads 2) – 2 independent lanes | $7,600.00 |
| NovaSeq 6000 S2 100 bp SR/50 PE flow cell | per cell (4.0 Billion reads 2) – 2 independent lanes | $9,800.00 |
| NovaSeq 6000 S2 100 bp PE flow cell | per cell (4.0 Billion reads 2) – 2 independent lanes | $11,800.00 |
| NovaSeq 6000 S2 150 bp PE flow cell | per cell (4.0 Billion reads 2) – 2 independent lanes | $12,500.00 |
| NovaSeq 6000 S4 100 bp PE flow cell | per cell (10.0 Billion reads 2) – 4 independent lanes | $16,650.00 |
| NovaSeq 6000 S4 150 bp PE flow cell | per cell (10.0 Billion reads 2) – 4 independent lanes | $18,500.00 |
1 For GSL library preparation, an additional charge for template quality & integrity will be added. Please see our Bioanalyzer & Tapestation pricing for more information.
2 Yield estimates reflect optimum values defined by Illumina and apply to GSL prepared libraries only. We cannot guarantee customer-supplied libraries will meet this estimate.
3 Lane or block pricing reflects divided user costs, however sequencing turn-around times are subject to sufficient user volume in order to fill the flow cell.
4 Block sequencing is performed on NovaSeq S4 150 PE lanes, and is only available for select GSL-prepared library options, we cannot offer block pricing options for user prepared libraries, or those not suitable for sequencing at 150×2 PE, or will impact the performance of other customer libraries .
PacBio Services
| Service | Unit | Price |
|---|---|---|
| HiFi Sample Size-Selection/Cleanup | per sample | $160.00 |
| HiFi DNA Library Prep (unbarcoded) | per sample | $450.00 |
| HiFi Barcoded DNA Library Prep | per sample | $475.00 |
| PacBio Iso-Seq Library Prep (1 fraction) | per sample | $500.00 |
| PacBio Sequel IIe Cell | per cell | $1,800.00 |
| PacBio Sequel IIe Cell (4 Cell Block) | per 4 cells | $6,000.00 |
Phage Sequencing
| Service | Unit | Price |
|---|---|---|
| Phage Sequencing | per sample | $225.00 |
| Phage Assembly | per sample | $50.00 |
Miscellaneous Services & Supplies
| Service | Unit | Price |
|---|---|---|
| Pippen Prep (1-3 samples) | per set | $83.00 |
| Pippen Prep ( 4-5 samples) | per set | $90.00 |
| BioRad CFX384 qPCR run 2,3 | per plate | $25.00 |
| BioRad digital droplet PCR 3 | per sample | $3.75 |
| Misc service | per hour | $80.00 |
| Robotics/instrumentation use 1 | per hour 1 | $38.00 |
| BigDye Sanger mix (600 uL) | per tube | $1,250.00 |
| Ampure XP Beads (10 mL) | per tube | $290.00 |
| Covaris G Tubes | per tube | $33.00 |
| Covaris Tubes | each | $8.25 |
| qPCR plate & seal for CFX384 2 | each | $7.00 |
| qPCR seal only 2 | each | $2.00 |
| Illumina Library Kit | Internal Users only, contact for quote. |
1 Usage fee applies to the Covaris Ultrasonicator, Qiagen TissueLyzer, Synergy H1 Hybrid Plate Reader, and GenoGrinder 2010 instruments. Users are charged in increments of 0.25 hours, depending on total use time.
2 When running the CFX384 qPCR machine, the user must use BioRad optical seals and qPCR plates. Please contact us before purchasing your own supplies for qPCR to ensure you are using the correct type.
3 This is shared research equipment and available only to local users. The GSL does not perform assays on this equipment as a direct service.