Note: All prices below are for all researchers, including those affiliated with NCSU, UNC, Duke, and other federally-funded or non-profit organizations. A standard 3% charge will be added for credit card transactions.

The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. Please contact the GSL Director BEFORE submitting ANY samples or research projects related to the SARS-COV2 pandemic or other samples of pathogenic risk to humans. We will try and point you to a suitable provider if we are unable to provide services directly.

Current GSL Services & Pricing (effective April 15th, 2022)

Coming Soon! 10X Genomics 3′ Single Cell Library Prep services 

Notes: 10X library prep is currently only available for internal users, utilizing cells with BSL1 (nonpathogenic) status. Due to unique cycle settings, 10X libraries require a dedicated flow cell for sequencing. Currently only raw .bcl file delivery available due to specific demultiplexing requirements.

Sanger Sequencing & Genotyping

96-well plate

ServiceUnitPrice
Genotyping & Fragment Analysis96 well plate $38.00
Platinum Sequencing - PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run96 well plate $365.00
Gold Sequencing - Seq Rxn, Clean Up & Capillary Run96 well plate $290.00
Bronze Sequencing - Clean Up & Capillary Run96 well plate $130.00

Single tube

ServiceUnitPrice
Platinum Sequencing - PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Runper sample$6.00
Gold Sequencing - Seq Rxn, Clean Up & Capillary Runper sample$4.50
Bronze Sequencing - Clean Up & Capillary Runper sample$2.75

Next-Generation Sequencing

Illumina Services

ServiceUnitPricing
Illumina DNA and RNA Libraries 1-7 per sample 1$150.00
Illumina DNA and RNA Libraries 8-15 per sample 1$140.00
Illumina DNA and RNA Libraries 16-89 per sample 1$120.00
Illumina DNA and RNA Libraries 90+ per sample 1$100.00
Illumina small RNA Librariesper sample 1$250.00
Hi-C Library prep (PhaseGenomics)per sample 1$925.00
16S Amplicon Library Prepper block of 48 or 96 samples$800.00 (1-48) or $1,600.00 (49-96)
10X Genomics 3' SC RNA library prep (internal users only, BSL1 only)first sample $2,300.00
10X Genomics 3' SC RNA library prep (internal users only, BSL1 only)per sample (samples 2-8)$1,900.00
MiSeq v2 250 PE NANO flow cellper cell (1M reads 2)$675.00
MiSeq v2 150 bp PE flow cellper cell (12M reads 2)$1,425.00
MiSeq v2 250 bp PE flow cellper cell (12M reads 2)$1,575.00
MiSeq v3 150 bp SR/ 75 bp PE flow cellper cell (20M reads 2)$1,275.00
MiSeq v3 300 bp PE flow cellper cell (20M reads 2)$1,950.00
NextSeq 500 v2 75 bp SR flow cellper cell (400M reads 2)$2,300.00
NextSeq 500 v2 150 bp SR/ 75 bp PE flow cellper cell (400M reads 2)$3,855.00
NextSeq 500 v2 150 bp PE flow cellper cell (400M reads 2)$5,785.00
Per sample Sequencing (NovaSeq S4 150 PE)per sample (40 Million reads3,4)$100.00
NovaSeq 6000 SP 100 bp SR lane ***per lane (400M reads 2,3)$2,050.00
NovaSeq 6000 SP 150 bp PE lane ***per lane (400M reads 2,3)$2,550.00
NovaSeq 6000 SP 100 bp SR/50 bp PE flow cellper cell (800M reads 2) - 2 independent lanes$4,100.00
NovaSeq 6000 S4 150 bp PE lane ***per lane (2.5 Billion reads 2,3)$4,500.00
NovaSeq 6000 SP 100 bp PE flow cellper cell (800M reads 2) - 2 independent lanes$4,750.00
NovaSeq 6000 SP 150 bp PE flow cellper cell (800M reads 2) - 2 independent lanes$5,100.00
NovaSeq 6000 SP 250 bp PE flow cellper cell (800M reads 2) - 2 independent lanes$5,700.00
NovaSeq 6000 S1 100 bp SR/50 PE flow cellper cell (1.6 Billion reads 2) - 2 independent lanes$6,000.00
NovaSeq 6000 S1 100 bp PE flow cellper cell (1.6 Billion reads 2) - 2 independent lanes$7,075.00
NovaSeq 6000 S1 150 bp PE flow cellper cell (1.6 Billion reads 2) - 2 independent lanes$7,500.00
NovaSeq 6000 S2 100 bp SR/50 PE flow cellper cell (4.0 Billion reads 2) - 2 independent lanes$9,600.00
NovaSeq 6000 S2 100 bp PE flow cellper cell (4.0 Billion reads 2) - 2 independent lanes$11,500.00
NovaSeq 6000 S2 150 bp PE flow cellper cell (4.0 Billion reads 2) - 2 independent lanes$12,150.00
NovaSeq 6000 S4 100 bp PE flow cellper cell (10.0 Billion reads 2) - 4 independent lanes$16,000.00
NovaSeq 6000 S4 150 bp PE flow cellper cell (10.0 Billion reads 2) - 4 independent lanes$17,700.00

1 For GSL library preparation, an additional charge for template quality & integrity will be added. Please see our Bioanalyzer & Tapestation pricing for more information.

2 Yield estimates reflect optimum values defined by Illumina and apply to GSL prepared libraries only. We cannot guarantee customer-supplied libraries will meet this estimate.

3 Lane or block pricing reflects divided user costs, however sequencing turn-around times are subject to sufficient user volume in order to fill the flow cell.

4 Block sequencing is performed on NovaSeq S4 150 PE lanes, and is only available for select GSL-prepared library options, we cannot offer block pricing options for user prepared libraries, or those not suitable for sequencing at 150×2 PE .

PacBio Services

ServiceUnitPrice
PacBio Library Prep (4-10 kb)per sample$400.00
PacBio Library Prep (15-20 kb)per sample$475.00
PacBio Microbial Indexed Library Prepper sample$275.00 (caveats apply, contact GSL for more information)
PacBio Iso-Seq Library Prep (1 fraction)per sample$500.00
PacBio Sequel I Cells (Block of 8)
per cell$1,200.00
PacBio Sequel I Cells (Block of 4)
per cell$1,250.00
PacBio Sequel I Cells (Block of 2)
per cell$1,300.00
PacBio Sequel I Cell
per cell$1,350.00

Phage Sequencing

ServiceUnitPrice
Phage Sequencingper sample$225.00
Phage Assemblyper sample$50.00

Miscellaneous Services & Supplies

ServiceUnitPrice
RNA (extraction from tissues)per 2 samples$85.00
TapeStation DNA Analysis (D1000/D5000 Tapes)per 12 samples 1$64.00
TapeStation High Sensitivity DNA Analysisper 12 samples$90.00
TapeStation High Molecular Weight DNA Analysisper 12 samples 1$90.00
BioAnalyzer RNA Analysis - Pico sensitivityper 11 samples 1$56.00
BioAnalyzer RNA Analysis - Nano sensitivityper 12 samples 1$68.00
Pippen Prep (1-3 samples)per set$78.00
Pippen Prep ( 4-5 samples)per set$82.00
BioRad CFX384 qPCR runper plate$25.00
BioRad digital droplet PCRper sample$3.25
Misc serviceper hour$80.00
Robotics/instrumentation use 3per hour 3$38.00
BigDye Sanger mix (600 uL)per tube$1,158.00
Ampure XP Beads (10 mL)per tube$275.00
Covaris G Tubesper tube$33.00
Covaris Tubeseach$7.00
qPCR plate & seal for CFX384each$7.00
qPCR seal only 2each$2.00
Illumina Library KitInternal Users only, contact for quote.

1 Split pricing is available for half (1-6) and full (7-12) sets of samples. Bioanalyzer RNA chips run 11-12 samples, depending on the sensitivity level (pico vs nano).

2 When running the CFX384 qPCR machine, the user must use BioRad optical seals and qPCR plates. Please contact us before purchasing your own supplies for qPCR to ensure you are using the correct type.

3 Usage fee applies to the Covaris S220 Ultrasonicator, Qiagen TissueLyser, and Synergy H1 Hybrid Plate Reader. Users are charged in increments of 0.25 hours, depending on total use time.