The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. Please contact the GSL Director BEFORE submitting ANY samples of pathogenic risk to humans . We will point you to a suitable provider if we are unable to provide services directly.
Nucleic Acid Isolation
Service Total Cost HT Plant DNA Isolation 96w plate $383.32 Kingfisher Nucleic Acid Isolation (Per Run) $100.00
Sample Processing
Sample QC
Service Total Cost TapeStation DNA/RNA Per Sample $10.06 Qubit $22.56 Picogreen Quant (96) $101.53
Automated Sample Handling
Service Total Cost Sample Normalization (96 samples) $39.34 Sample Pooling (96 samples) $31.72 Instrument Use $59.34
Next-Generation Sequencing – Illumina Services
Library Prep
Service Total Cost RNA Library Prep HT- PolyA (8 Samples) $483.05 RNA Library Prep HT- Ribodep (8 Samples) $532.05 Illumina Small RNA Library Prep $102.65 Illumina DNA Library Prep HT (8 samples) $428.61 Phage Library Prep $61.80 Hi-C NGS Library Prep $755.33 16S/ITS Amplicon Library Prep (96 samples) $1,838.37 HIVE Single Cell RNAseq Library Prep $1,385.54 Cut&Run Library Prep $61.16
Pricing is per sample unless otherwise stated. MiSeq Sequencing Options
Service Total Cost MiSeq v2 150 PE $1,733.15 MiSeq v2 250 PE $1,911.79 MiSeq v3 150 SR $1,542.40 MiSeq v3 300 PE $2,398.25 MiSeq v2 Nano 250 PE $778.39 MiSeq v2 50 SR $1,412.89 MiSeq v2 Micro 150 PE $1,733.15 MiSeq v2 Nano 150 PE $703.28
NextSeq 2000 Sequencing Options
Service Total Cost NextSeq 2000 P1 100SE/50PE $1,277.41 NextSeq 2000 P1 150 PE $1,641.40 NextSeq 2000 P1 300 PE $2,274.53 NextSeq 2000 P2 100 SE/50 PE $1,794.57 NextSeq 2000 P2 100 PE $2,922.23 NextSeq 2000 P2 150 PE $3,772.45 NextSeq 2000 P2 300 PE $4,142.71 NextSeq 2000 P3 100 SE/50 PE $3,235.10 NextSeq 2000 P3 100 PE $4,341.00 NextSeq 2000 P3 150 PE $5,647.63 NextSeq 2000 P4 50SE/25PE $2,238.52 NextSeq 2000 P4 100SE/50PE $3,247.78 NextSeq 2000 P4 100PE $4,501.51 NextSeq 2000 P4 150PE $5,970.00
NovaSeq X Sequencing Options
Service Total Cost Outsource sequencing $149.00
The GSL now offers sequencing on the NovaSeq X platform. For flowcell options and pricing, please contact us directly: gsl_support@ncsu.edu . Next-Generation Sequencing – PacBio Services
Service Total Cost HiFi DNA Library Prep (15-20kb) $287.20 Indexed HiFi DNA Prep $294.70 PacBio DNA Cleanup $67.76 PacBio Sequel IIe SMRT cell (10-hour run) $2,325.62 PacBio Sequel IIe SMRT cell (30-hour run) $2,425.62
Other Services and Supplies
Service Total Cost QPCR Plate Run $30.00 Digital Droplet PCR (Per Sample) $5.25 Shared User Equipment $38.00 Employee Effort $38.00 Phage Assembly $50.00 Ampure XP Beads (10 mL) ———- qPCR plate & seal for CFX384 ———-
Includes: Covaris, Genogrinder, Blue Pippin, Tissue Lyzer, Plate Reader, KASP station. Pricing is per hour.