A Financial Agreement (internal users) or Services Order Form (external users) must be in place before services commence
All PIs are responsible for maintaining active financial agreements in MENDIX, including setting up agreements for new grant awards and adding and removing participants as the research group changes over time
All projects must be initiated as a consultation request in the Lab Management Portal including requests to become an expert or applications superuser
Any samples requiring BSL2 and above handling procedures must be accompanied by an Exposure Control Plan
Calibration solutions, MS grade solvents, solvent bottles, and autosampler vials will be provided by METRIC. All other consumable must be provided by or charged to the user
METRIC does not allow the use of any solvents below MS grade, ion pairing reagents, or any unusual modifiers without permission
METRIC provides all gas cylinders required to operate instrumentation; only METRIC staff and expert superusers are permitted to change out gas cylinders
Google Drive and Dropbox are the only allowed data transfer mechanisms
No External Hard Drives or Flash Drives, Web Surfing or Program Downloads on Data Acquisition Computers
No remote access to data acquisition computers outside of your instrument reservation period
Weekly restart for update installation required for all data acquisition computers
Licensed data analysis software (Compound Discoverer, Proteome Discoverer, Lipid Search) is for METRIC staff use only
Training of clients on open source data analysis software or purchased copies of licensed software will be approved on a case by case basis for METRIC collaborators only
All samples submitted to METRIC must be labeled clearly and include a detailed description of what each sample ID is as well as any protocols for processing that the samples were subjected to; samples will be stored by METRIC for no longer than 6 months after project completion.
METRIC will provide raw data files to clients for transfer to their own storage device; METRIC will store raw and processed data for a maximum of 1 year post-analysis, after which METRIC assumes no responsibility for data storage.
All users agree to cite METRIC in any publications or grant proposals for which data collected in METRIC is used for
Authorship of scientific papers should include individuals who make meaningful and substantive contributions to the intellectual content of the work to be published.
Qualification: Instrument is predominantly used by a single research group; usage must exceed 50% max usage
Training: PI and/or research group is present at installation or pays for training by instrument engineers; research group is thereafter responsible for training new group members
Reservation Policies: research group is responsible for mass and system calibration before usage and system suitability tests before and after usage and maintenance; minimum instrument reservations are 4 h for MALDESI and ICP-MS and 8 h for Orbitrap, TOF, and QqQ instruments; for multi-day reservations, the instrument should be reserved overnight (24 h blocks) with the exception of MALDESI and ICP-MS which should be reserved in 8 h blocks
Routine Maintenance: routine maintenance for all MS (e.g. system calibration, ITT and sweep cone cleaning) and LC (check valve sonication, purging and flushing of all mobile phase lines) components should be performed by the superuser research group
Service Calls: service calls should be documented using the METRIC google form
Emergency Procedures: in the event of a power outage (planned or unplanned), the superuser research group will be responsible for any necessary shut down and start up procedures and will report system status to METRIC
Qualification: PI requests students independently operate METRIC MS instrumentation and expects a at least 8 week per year of instrument usage per application
Training: Initial training by METRIC staff at the assisted use hourly rate, typically consisting of three 6h training days; training should be limited to two main users per research group; training of new students upon graduation of a trained user shall also be performed by METRIC staff at the assisted hourly rate; any METRIC assistance requested after training will also be charged.
Set-up Fees: set-up fee of $300 prior to all instrument reservations (setup fee includes ITT and sweep cone cleaning, mass calibration and system suitability assessment)
Reservation Policies: minimum instrument reservations are 4 h for ICP-MS and 8 h for Orbitrap, TOF, and QqQ instruments; for multi-day reservations, the instrument should be reserved overnight (24 h blocks) with the exception of proteomics (48 h blocks) and ICP-MS (8 h blocks)
Routine Maintenance: METRIC is responsible for routine maintenance for all MS (system calibration, ITT and sweep cone cleaning) and LC (check valve sonication, purging and flushing of all mobile phase lines) components
Service Calls: service calls cannot be initiated by an applications superuser and should be should be communicated to METRIC staff by documentation using the METRIC google form
Emergency Procedures: in the event of a power outage (planned or unplanned), METRIC will be responsible for any necessary shut down and start up procedures
All sample preparation equipment is for use by METRIC personnel only; including but not limited to multichannel and repeater pipettes, vacuum concentrators, centrifuges, geno grinders, bead disruptors, tissue homogenizers, pH meters, positive pressure manifolds, N2 evaporation manifolds, SPE vacuum manifolds, water baths, sonicating baths and high purity water stations
METRIC pipettes and balances are calibrated twice annually
Sample storage freezers are on generator power and serviced annually
Water purification systems are serviced annually
Speed-vacs have oil-free pumps and are maintained with coolant and routine cleaning of the solvent trap
Positive pressure manifolds and N2 evaporation units are only supplied with grade 5 N2
SPE vacuum manifolds are rinsed and stored properly after each use; replacement parts are kept on hand and when used are reordered to maintain inventory
All sample preparation should take place in the superuser lab and fully prepped samples only should be brought to the ICP-MS instrumentation laboratory
Samples should be as clean as possible and filtered using a ca. 1 μm filter
Concentrated strong acids (>30% v/v) should not be handled or stored around the instrument or in the instrument laboratory (i.e., HCl, HNO3)
Superusers and group members should run a Performance Report before and after their analysis to document instrument performance