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blue dna

Pricing

Note: All prices below are for NC State University faculty and research investigators. For external customers, please reach out (Contact Page) for appropriate pricing and quotes. A standard 3% charge will be added for credit card transactions.

The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. Please contact the GSL Director BEFORE submitting ANY samples of pathogenic risk to humans. We will point you to a suitable provider if we are unable to provide services directly.

GSL Services & Pricing (effective May 30th, 2024)

Nucleic Acid Isolation & Quantification

Note: GSL DNA extraction services may not be suitable for NGS applications requiring extremely high molecular weight DNA inputs, such as PacBio or Oxford Nanopore long-read sequencing approaches.

ServiceUnitPrice
RNA Extractionsper 2 samples$100.00
HT Plant DNA extraction (Oktopure)per 96w plate$350.00
HT Plant DNA extraction (Oktopure)per sample$3.75
DNA extractions (Kingfisher) per 96 samples$550.00
LT DNA extractions (Kingfisher)
from 1-24 samples$225.00
FS Picogreen DNA Quant (384)from 97-384 samples$275.00
FS Picogreen DNA Quant (96)from 49-96 samples$130.00
FS Picogreen DNA Quant (48)from 1- 48 samples$95.00
Sample Normalization (96)per plate$34.00
TapeStation DNA Analysis (D1000/D5000 Tapes)per 12 samples 1$68.00
TapeStation High Sensitivity DNA Analysisper 12 samples 1$96.00
TapeStation High Molecular Weight DNA Analysisper 12 samples 1$96.00
TapeStation RNA Analysisper 12 samples 1$65.00
TapeStation RNA HS Analysisper 12 samples 1$70.00

1 Split pricing is available for half (1-6) and full (7-12) sets of samples.

Sanger Sequencing & Genotyping

96-well plate

ServiceUnitPrice
Genotyping & Fragment Analysis96 well plate$76.00
Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run96 well plate$435.00
Gold Sequencing – Seq Rxn, Clean Up & Capillary Run96 well plate$345.00
Bronze Sequencing – Clean Up & Capillary Run96 well plate$162.00

Single tube

ServiceUnitPrice
Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Runper sample$6.50
Gold Sequencing – Seq Rxn, Clean Up & Capillary Runper sample$5.00
Bronze Sequencing – Clean Up & Capillary Runper sample$3.00

Next-Generation Sequencing

Illumina Services

ServiceUnitPricing
Illumina-based Library Prep Services
Illumina DNA Library Prep LT (1-12 tier)per sample 1 $110.00
Illumina DNA Library Prep LT (12+ tier)per sample 1 $98.00
Illumina HT DNA Library Prep (96)per 96w plate$5,000.00
NEB RNA Library Prep PolyA (1-12 tier)per sample 1$142.00
NEB RNA Library Prep PolyA (13-48 tier)per sample 1$140.00
NEB RNA Library Prep PolyA (48+ tier)per sample 1$138.00
NEB RNA Library Prep with Ribodepletionper sample 1$212.00
Illumina small RNA Library Prepper sample 1$250.00
Hi-C Library prep (PhaseGenomics)per sample 1$1,000.00
16S v3/v4 or ITS1 Amplicon Library Prepper block of 48 or 96 reactions*$965.00 (1-48) or $1,930.00 (49-96)
10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only)first sample$2,700.00
10X Genomics 3′ SC RNA library prep (internal users only, BSL1 only)per sample (samples 2-8)$2,100.00
MiSeq Sequencing Options
MiSeq v2 250 PE NANO flow cellper cell (1M reads 2)$795.00
MiSeq v2 150 bp PE flow cellper cell (12M reads 2)$1,650.00
MiSeq v2 250 bp PE flow cellper cell (12M reads 2)$1,800.00
MiSeq v3 150 bp SR/ 75 bp PE flow cellper cell (20M reads 2)$1,425.00
MiSeq v3 300 bp PE flow cellper cell (20M reads 2)$2,250.00
NextSeq 2000 Sequencing Options
NextSeq 100SR/ 50 PE P1 flow cellper cell (100M reads 2)$1,200.00
NextSeq 150 PE P1 flow cellper cell (100M reads 2)$1,525.00
NextSeq 300 PE P1 flow cellper cell (100M reads 2)$2,175.00
NextSeq 100SR/ 50 PE P2 flow cellper cell (400M reads 2)$1,550.00
NextSeq 100 PE P2 flow cellper cell (400M reads 2)$2,900.00
NextSeq 150 PE P2 flow cellper cell (400M reads 2)$3,745.00
NextSeq 300 PE P2 flow cellper cell (400M reads 2)$4,050.00
NextSeq 50SR/ 25 PE P3 flow cellper cell (1.2 Billion reads 2)$2,350.00
NextSeq 100SR/ 50 PE P3 flow cellper cell (1.2 Billion reads 2)$3,350.00
NextSeq 100 PE P3 flow cellper cell (1.2 Billion reads 2)$4,600.00
NextSeq 150 PE P3 flow cellper cell (1.2 Billion reads 2)$6,150.00
New! NextSeq 50SR/ 25 PE P4 XLEAP flow cellper cell (1.8 Billion reads 2)$1,925.00
New! NextSeq 100SR/ 50 PE P4 XLEAP flow cellper cell (1.8 Billion reads 2)$2,875.00
New! NextSeq 100 PE P4 XLEAP flow cellper cell (1.8 Billion reads 2)$4,035.00
New! NextSeq 150 PE P4 XLEAP flow cellper cell (1.8 Billion reads 2)$5,125.00
NovaSeq 6000 Sequencing Options
Best Deal! Per Sample Sequencing (150 PE)per sample (40 Million reads3,4)$125.00
Best Deal! NovaSeq S4 150 bp PE laneper lane (2.5 Billion reads 2,3)$4,500.00
NovaSeq SP 100 SR/50 PE flow cellper cell (800M reads 2) – 2 independent lanes$4,000.00
NovaSeq SP 100 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$4,775.00
NovaSeq SP 150 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$5,100.00
NovaSeq SP 250 bp PE flow cellper cell (800M reads 2) – 2 independent lanes$6,000.00
NovaSeq S1 100 bp SR/50 PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$6,100.00
NovaSeq S1 100 bp PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$7,300.00
NovaSeq S1 150 bp PE flow cellper cell (1.6 Billion reads 2) – 2 independent lanes$7,775.00
NovaSeq S2 100 bp SR/50 PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$10,150.00
NovaSeq S2 100 bp PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$12,250.00
NovaSeq S2 150 bp PE flow cellper cell (4.0 Billion reads 2) – 2 independent lanes$13,000.00
NovaSeq S4 100 bp PE flow cellper cell (10.0 Billion reads 2) – 4 independent lanes$15,775.00
NovaSeq S4 150 bp PE flow cellper cell (10.0 Billion reads 2) – 4 independent lanes$17,500.00

1 For GSL library preparation, an additional charge for template quality & integrity will be added. Please see our  Tapestation pricing for more information.

2 Yield estimates reflect optimum values defined by Illumina and apply to GSL prepared libraries only. We cannot guarantee customer-supplied libraries will meet this estimate.

Lane or block pricing reflects divided user costs, however sequencing turn-around times are subject to sufficient user volume in order to fill the flow cell.

Block sequencing is performed on NovaSeq S4 150 PE lanes, and is only available for select GSL-prepared library options, we cannot offer block pricing options for user prepared libraries, or those not suitable for sequencing at 150×2 PE, or require run settings that will impact the performance of other customer libraries.

Reaction is defined as a single amplicon NGS prep for a given sample, such as 16S or ITS. The GSL cannot prep multiple amplicons together within the same sample reaction (each is discretely barcoded per our standard protocol).

PacBio Services

ServiceUnitPrice
HiFi Sample Size-Selection/Cleanupper sample$165.00
HiFi DNA Library Prep (unbarcoded)per sample$375.00
HiFi Barcoded DNA Library Prepper sample$400.00
PacBio Iso-Seq Library Prep (1 fraction)per sample$500.00
PacBio Sequel IIe Cell per cell$2,225.00
PacBio Sequel IIe Cell (4 Cell Block)per 4 cells$7,625.00

Phage Sequencing

ServiceUnitPrice
Phage Sequencingper sample$225.00
Phage Assemblyper sample$50.00

Miscellaneous Services & Supplies

ServiceUnitPrice
Pippen Prep (1-3 samples)per set$85.00
Pippen Prep ( 4-5 samples)per set$92.00
BioRad CFX384 qPCR run 2,3per plate$28.00
BioRad digital droplet PCR 3per sample$4.25
Misc serviceper hour$85.00
Robotics/instrumentation use 1per hour 1$40.00
BigDye Sanger mix (600 uL)per tube$1,325.00
Ampure XP Beads (10 mL)per tube$310.00
Covaris Tubeseach$7.50
qPCR plate & seal for CFX384 2each$7.00
qPCR seal only 2each$2.00
Illumina Library KitInternal Users only, contact for quote.

1 Usage fee applies to the Covaris Ultrasonicator, Qiagen TissueLyzer, Synergy H1 Hybrid Plate Reader, and GenoGrinder 2010 instruments. Users are charged in increments of 0.25 hours, depending on total use time.

2 When running the CFX384 qPCR machine, the user must use BioRad optical seals and qPCR plates. Please contact us before purchasing your own supplies for qPCR to ensure you are using the correct type.

3 This is shared research equipment and available only to local users. The GSL does not perform assays on this equipment as a direct service.